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ENZYME

ENZYME entry: EC 1.14.15.15

Accepted Name
cholestanetriol 26-monooxygenase
Alternative Name(s)
5beta-cholestane-3alpha,7alpha,12alpha-triol 26-hydroxylase
5beta-cholestane-3alpha,7alpha,12alpha-triol hydroxylase
cholestanetriol 26-hydroxylase
cholesterol 27-hydroxylase
cytochrome P450 27A1'
sterol 26-hydroxylase
sterol 27-hydroxylase
vitamin D3 25-hydroxylase
Reaction catalysed
5beta-cholestane-3alpha,7alpha,12alpha-triol + 5 H(+) + 3 O2 + 6 reduced [adrenodoxin] <=> (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + 4 H2O + 6 oxidized [adrenodoxin]
Comment(s)
  • Catalyzes the first three sterol side chain oxidations in bile acid biosynthesis via the neutral (classic) pathway.
  • Can also act on cholesterol, cholest-5-en-3beta,7alpha-diol, 7alpha- hydroxycholest-4-en-3-one, and 5beta-cholestane-3alpha,7alpha-diol.
  • The enzyme can also hydroxylate cholesterol at positions 24 and 25.
  • The initial source of the electrons is NADPH, which transfers the electrons to the adrenodoxin via EC 1.18.1.6.
  • Formerly EC 1.14.13.15.
Cross-references
BRENDA1.14.15.15
EC2PDB1.14.15.15
ExplorEnz1.14.15.15
PRIAM enzyme-specific profiles1.14.15.15
KEGG Ligand Database for Enzyme Nomenclature1.14.15.15
IUBMB Enzyme Nomenclature1.14.15.15
IntEnz1.14.15.15
MEDLINEFind literature relating to 1.14.15.15
MetaCyc1.14.15.15
Rhea expert-curated reactions1.14.15.15
UniProtKB/Swiss-Prot
Q02318, CP27A_HUMANQ9DBG1, CP27A_MOUSEP17177, CP27A_RABIT
P17178, CP27A_RATC4B644, CPVDH_PSEAH

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