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ENZYME entry: EC

Accepted Name
cholest-4-en-3-one 26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming].
Alternative Name(s)
cholest-4-en-3-one 26-monooxygenase.
cholest-4-en-3-one 27-monooxygenase.
Reaction catalysed
cholest-4-en-3-one + 5 H(+) + 3 O2 + 6 reduced [2Fe-2S]-[ferredoxin] <=> (25S)-3-oxocholest-4-en-26-oate + 4 H2O + 6 oxidized [2Fe-2S]-[ferredoxin]
  • A cytochrome P450 (heme-thiolate) protein found in several bacterial pathogens.
  • The enzyme is involved in degradation of the host's cholesterol.
  • It catalyzes the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol.
  • The products are exclusively in the (25S) conformation.
  • The enzyme also accepts cholesterol as a substrate.
  • Cf. EC
  • Formerly EC
PRIAM enzyme-specific profiles1.14.15.29
KEGG Ligand Database for Enzyme Nomenclature1.14.15.29
IUBMB Enzyme Nomenclature1.14.15.29
MEDLINEFind literature relating to
Rhea expert-curated reactions1.14.15.29
P63710, CP125_MYCBO;  A0R4Y3, CP125_MYCS2;  P9WPP0, CP125_MYCTO;  
P9WPP1, CP125_MYCTU;  Q0S7M1, CP125_RHOJR;  

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