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ENZYME

ENZYME entry: EC 1.14.15.29

PURL: https://purl.expasy.org/enzyme/EC/1.14.15.29
Accepted Name
cholest-4-en-3-one 26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming]
Alternative Name(s)
cholest-4-en-3-one 26-monooxygenase
cholest-4-en-3-one 27-monooxygenase
CYP125
CYP125A1
Reaction catalysed
cholest-4-en-3-one + 6 reduced [2Fe-2S]-[ferredoxin] + 3 O2 + 5 H(+) = (25S)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S]-[ferredoxin] + 4 H2O
Comment(s)
  • A cytochrome P450 (heme-thiolate) protein found in several bacterial pathogens.
  • The enzyme is involved in degradation of the host's cholesterol.
  • It catalyzes the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol.
  • The products are exclusively in the (25S) conformation.
  • The enzyme also accepts cholesterol as a substrate. cf. EC 1.14.15.28.
  • Formerly EC 1.14.13.141.
Cross-references
BRENDA1.14.15.29
EC2PDB1.14.15.29
ExplorEnz1.14.15.29
KEGG Ligand Database for Enzyme Nomenclature1.14.15.29
IUBMB Enzyme Nomenclature1.14.15.29
MEDLINEFind literature relating to 1.14.15.29
MetaCyc1.14.15.29
Rhea expert-curated reactions1.14.15.29
UniProtKB/Swiss-Prot
P63710, CP125_MYCBOA0R4Y3, CP125_MYCS2P9WPP0, CP125_MYCTO
P9WPP1, CP125_MYCTUQ0S7M1, CP125_RHOJR

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