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ENZYME entry: EC 1.14.15.29

Accepted Name
Cholest-4-en-3-one 26-monooxygenase ((25S)-3-oxocholest-4-en-26-oate forming).
Alternative Name(s)
Cholest-4-en-3-one 26-monooxygenase.
Cholest-4-en-3-one 27-monooxygenase.
CYP125.
CYP125A1.
Reaction catalysed
Cholest-4-en-3-one + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H(+) + 3 O(2) <=> (25S)-3-oxocholest-4-en-26-oate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H(2)O
Cofactor(s)
Heme-thiolate.
Comment(s)
  • A cytochrome P450 (heme-thiolate) protein found in several bacterial pathogens.
  • The enzyme is involved in degradation of the host's cholesterol.
  • It catalyzes the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol.
  • The products are exclusively in the (25S) conformation.
  • The enzyme also accepts cholesterol as a substrate.
  • Cf. EC 1.14.15.28.
  • Formerly EC 1.14.13.141.
Cross-references
BRENDA1.14.15.29
EC2PDB1.14.15.29
ExplorEnz1.14.15.29
PRIAM enzyme-specific profiles1.14.15.29
KEGG Ligand Database for Enzyme Nomenclature1.14.15.29
IUBMB Enzyme Nomenclature1.14.15.29
IntEnz1.14.15.29
MEDLINEFind literature relating to 1.14.15.29
MetaCyc1.14.15.29
Rhea expert-curated reactions1.14.15.29
UniProtKB/Swiss-Prot
P63710, CP125_MYCBO;  A0R4Y3, CP125_MYCS2;  P9WPP0, CP125_MYCTO;  
P9WPP1, CP125_MYCTU;  Q0S7M1, CP125_RHOJR;  

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