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ENZYME

ENZYME entry: EC 1.14.19.18

PURL: https://purl.expasy.org/enzyme/EC/1.14.19.18
Accepted Name
sphingolipid 8-(E)-desaturase
Alternative Name(s)
8 fatty acid desaturase
8-sphingolipid desaturase
Reaction catalysed
an N-acylsphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = a (4E,8E)-4-sphinga-4,8-dienine ceramide + 2 Fe(III)-[cytochrome b5] + 2 H2O
Comment(s)
  • The enzyme, characterized from the yeasts Kluyveromyces lactis and Candida albicans and from the diatom Thalassiosira pseudonana, introduces a trans double bond at the 8-position of sphingoid bases in sphingolipids.
  • The enzyme determines the position of the double bond by its distance from the alcohol end of the sphingoid base, and contains a cytochrome b5 domain that acts as the direct electron donor to the active site of the desaturase.
  • The homologous enzymes from higher plants, EC 1.14.19.29, act on phytosphinganine (4-hydroxysphinganine) and produces a mixture of trans and cis isomers.
Cross-references
BRENDA1.14.19.18
EC2PDB1.14.19.18
ExplorEnz1.14.19.18
KEGG Ligand Database for Enzyme Nomenclature1.14.19.18
IUBMB Enzyme Nomenclature1.14.19.18
MEDLINEFind literature relating to 1.14.19.18
MetaCyc1.14.19.18
Rhea expert-curated reactions1.14.19.18
UniProtKB/Swiss-Prot
C8V7U7, SDEA_EMENIQ5AEK8, SLD1_CANALQ6CMK7, SLD1_KLULA
Q8NKG8, SLD1_KLULCC4QVU3, SLD1_KOMPGQ8NKG9, SLD1_LACK1

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