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ENZYME entry: EC 1.14.99.67

Accepted Name
alpha-N-dichloroacetyl-p-aminophenylserinol N-oxygenase
Reaction catalysed
AH2 + alpha-N-dichloroacetyl-p-aminophenylserinol + 2 O2 = A + chloramphenicol + 2 H2O
Comment(s)
  • The enzyme, isolated from the bacterium Streptomyces venezuelae, is involved in the biosynthesis of the antibiotic chloramphenicol.
  • It contains a carboxylate-bridged binuclear non-heme iron cluster.
  • The components of the native electron chain have not been identified, although the immediate donor is likely to be an iron-sulfur protein.
  • The reaction mechanism involves formation of an extremely stable peroxo intermediate that catalyzes three individual two-electron oxidations via a hydroxylamine and a nitroso intermediates without releasing the intermediates. cf. EC 1.14.99.68.
Cross-references
BRENDA1.14.99.67
EC2PDB1.14.99.67
ExplorEnz1.14.99.67
PRIAM enzyme-specific profiles1.14.99.67
KEGG Ligand Database for Enzyme Nomenclature1.14.99.67
IUBMB Enzyme Nomenclature1.14.99.67
IntEnz1.14.99.67
MEDLINEFind literature relating to 1.14.99.67
MetaCyc1.14.99.67
Rhea expert-curated reactions1.14.99.67
UniProtKB/Swiss-Prot
F2RB83, CMLI_STRVP

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