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ENZYME entry: EC 1.18.6.1

Accepted Name
Nitrogenase.
Reaction catalysed
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O <=> 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Cofactor(s)
Iron-sulfur; Vanadium cation or Mo cation.
Comment(s)
  • The enzyme is a complex of two components (namely dinitrogen reducatse and dinitrogenase).
  • Dinitrogen reductase is a [4Fe-4S] protein, which, in the presence of two molecules of ATP, transfers an electron from ferredoxin to the dinitrogenase component.
  • Dinitrogenase is a molybdenum-iron protein that reduces dinitrogen to two molecules of ammonia in three successive two-electron reductions via diazene and hydrazine.
  • The reduction is initiated by formation of hydrogen in stoichiometric amounts.
  • Acetylene is reduced to ethylene (but only very slowly to ethane), azide to nitrogen and ammonia, and cyanide to methane and ammonia.
  • In the absence of a suitable substrate, hydrogen is slowly formed.
  • Ferredoxin may be replaced by flavodoxin (see EC 1.19.6.1).
  • The enzyme does not reduce CO (cf. EC 1.18.6.2).
  • Formerly EC 1.18.2.1.
Cross-references
PROSITEPDOC00085 ; PDOC00580
BRENDA1.18.6.1
EC2PDB1.18.6.1
ExplorEnz1.18.6.1
PRIAM enzyme-specific profiles1.18.6.1
KEGG Ligand Database for Enzyme Nomenclature1.18.6.1
IUBMB Enzyme Nomenclature1.18.6.1
IntEnz1.18.6.1
MEDLINEFind literature relating to 1.18.6.1
MetaCyc1.18.6.1
Rhea expert-curated reactions1.18.6.1
UniProtKB/Swiss-Prot
P16266, ANFD_AZOVI;  O68955, ANFD_HELGE;  Q07933, ANFD_RHOCA;  
Q46082, ANFD_RUMHU;  O68946, ANFG_AZOMA;  O68943, ANFG_AZOPA;  
P16268, ANFG_AZOVI;  Q46244, ANFG_CLOPA;  Q07934, ANFG_RHOCA;  
O68940, ANFG_RHORU;  Q46083, ANFG_RUMHU;  P16267, ANFK_AZOVI;  
Q07935, ANFK_RHOCA;  Q46084, ANFK_RUMHU;  P06662, NIFD_ACIFI;  
Q44045, NIFD_ALCFA;  P33177, NIFD_ANASL;  P25313, NIFD_AZOBR;  
P07328, NIFD_AZOVI;  P06121, NIFD_BRADU;  P06120, NIFD_BRASP;  
P00467, NIFD_CLOPA;  O07642, NIFD_CROS5;  Q02452, NIFD_FRAAL;  
P77874, NIFD_HERSE;  P00466, NIFD_KLEPN;  Q00239, NIFD_LEPBY;  
P55170, NIFD_METBA;  P51605, NIFD_METIV;  P0CW50, NIFD_METMI;  
P0CW51, NIFD_METMP;  O27605, NIFD_METTH;  P20620, NIFD_METTL;  
Q50788, NIFD_METTM;  P52337, NIFD_NOSCO;  P00464, NIFD_NOSS1;  
P06769, NIFD_RHICP;  P00465, NIFD_RHILT;  P08717, NIFD_RHOCA;  
Q55029, NIFD_RIPO1;  P19066, NIFD_SINFN;  P26251, NIFH1_AZOC5;  
P26248, NIFH1_AZOCH;  P00459, NIFH1_AZOVI;  P00456, NIFH1_CLOPA;  
Q47917, NIFH1_MASLA;  P54799, NIFH1_METBA;  P51602, NIFH1_METIV;  
O27602, NIFH1_METTH;  P25767, NIFH1_METTL;  Q50785, NIFH1_METTM;  
P00457, NIFH1_NOSS1;  Q9AKT8, NIFH1_PAEDU;  P0CY95, NIFH1_RHOCA;  
D5ANI3, NIFH1_RHOCB;  P0A3S1, NIFH1_TRIV2;  P26252, NIFH2_AZOC5;  
P06118, NIFH2_AZOCH;  P15335, NIFH2_AZOVI;  P09552, NIFH2_CLOPA;  
Q47921, NIFH2_MASLA;  P54800, NIFH2_METBA;  P08624, NIFH2_METIV;  
O26739, NIFH2_METTH;  P08625, NIFH2_METTL;  O30577, NIFH2_NOSS1;  
Q9AKT4, NIFH2_PAEDU;  Q07942, NIFH2_RHOCA;  Q44484, NIFH2_TRIV2;  
P16269, NIFH3_AZOVI;  P09553, NIFH3_CLOPA;  P22548, NIFH4_CLOPA;  
P09554, NIFH5_CLOPA;  P09555, NIFH6_CLOPA;  B7JA99, NIFH_ACIF2;  
B5ER76, NIFH_ACIF5;  P06661, NIFH_ACIFR;  Q44044, NIFH_ALCFA;  
A6TTY3, NIFH_ALKMQ;  P33178, NIFH_ANASL;  P17303, NIFH_AZOBR;  
B6YRI6, NIFH_AZOPC;  P06117, NIFH_BRADU;  P00463, NIFH_BRASP;  
A3PLS9, NIFH_CERS1;  Q3J0H1, NIFH_CERS4;  O31183, NIFH_CERSP;  
Q3AR70, NIFH_CHLCH;  B3EH88, NIFH_CHLL2;  Q3B2P6, NIFH_CHLL3;  
B3QQ12, NIFH_CHLP8;  B3EL81, NIFH_CHLPB;  A1BEH0, NIFH_CHLPD;  
A4SFU6, NIFH_CHLPM;  Q8KC92, NIFH_CHLTE;  Q97ME5, NIFH_CLOAB;  
O07641, NIFH_CROS5;  B8HWE3, NIFH_CYAP4;  Q47G67, NIFH_DECAR;  
Q3Z7C7, NIFH_DEHM1;  C0QKK8, NIFH_DESAH;  B8FAC4, NIFH_DESAL;  
B1I0Y8, NIFH_DESAP;  Q09158, NIFH_DESMC;  A4J8C2, NIFH_DESRM;  
P26249, NIFH_ENTAG;  P08925, NIFH_FRAAL;  Q2J4F8, NIFH_FRACC;  
Q47922, NIFH_FRASE;  A8L2C4, NIFH_FRASN;  P46034, NIFH_FRASP;  
B7KG76, NIFH_GLOC7;  Q9ZIE4, NIFH_GLUDA;  P77873, NIFH_HERSE;  
B5XPH2, NIFH_KLEP3;  P00458, NIFH_KLEPN;  Q00240, NIFH_LEPBY;  
A0L6X0, NIFH_MAGMM;  C6BX34, NIFH_MARSD;  P71156, NIFH_MEGGA;  
Q0W443, NIFH_METAR;  Q2FUB7, NIFH_METHJ;  Q58289, NIFH_METJA;  
Q8PYY0, NIFH_METMA;  P0CW56, NIFH_METMI;  A3CWW3, NIFH_METMJ;  
P0CW57, NIFH_METMP;  B8ITG7, NIFH_METNO;  B0UAK2, NIFH_METS4;  
P06119, NIFH_METVO;  P26250, NIFH_NOSCO;  Q51296, NIFH_NOSS6;  
P52336, NIFH_NOSSN;  Q6D2Y8, NIFH_PECAS;  B4SC59, NIFH_PELPB;  
B4S9H5, NIFH_PROA2;  A4VJ70, NIFH_PSEU5;  P00462, NIFH_RHIET;  
P20623, NIFH_RHILE;  Q98AP7, NIFH_RHILO;  P00461, NIFH_RHILT;  
P00460, NIFH_RHIME;  Q2J1I1, NIFH_RHOP2;  Q13C78, NIFH_RHOPS;  
P22921, NIFH_RHORU;  Q55028, NIFH_RIPO1;  A7NR80, NIFH_ROSCS;  
A5USK5, NIFH_ROSS1;  Q59270, NIFH_RUMCE;  P19068, NIFH_SINFN;  
C4XRK2, NIFH_SOLM1;  A0LH11, NIFH_SYNFM;  Q2JTL7, NIFH_SYNJA;  
Q2JP78, NIFH_SYNJB;  C5BTB0, NIFH_TERTT;  C4LAS5, NIFH_TOLAT;  
P0A3S2, NIFH_TRIAZ;  O34106, NIFH_TRIEI;  P26254, NIFH_TRITH;  
Q7M8U8, NIFH_WOLSU;  Q5NLG3, NIFH_ZYMMO;  P15052, NIFK_ACIFI;  
P25314, NIFK_AZOBR;  P07329, NIFK_AZOVI;  P20621, NIFK_BRADU;  
P06122, NIFK_BRASP;  P11347, NIFK_CLOPA;  O07643, NIFK_CROS5;  
Q57118, NIFK_FRAAL;  P77875, NIFK_HERSE;  P09772, NIFK_KLEPN;  
P51754, NIFK_METBA;  P0CW52, NIFK_METMI;  P0CW53, NIFK_METMP;  
O27606, NIFK_METTH;  P20622, NIFK_METTL;  P95296, NIFK_METTM;  
P00468, NIFK_NOSS1;  Q55030, NIFK_RIPO1;  P19067, NIFK_SINFN;  
P15332, VNFD_AZOCH;  O68953, VNFD_AZOPA;  O68954, VNFD_AZOSA;  
P16855, VNFD_AZOVI;  P15333, VNFG_AZOCH;  O68951, VNFG_AZOSA;  
P16857, VNFG_AZOVI;  P15334, VNFK_AZOCH;  O68949, VNFK_AZOPA;  
O68952, VNFK_AZOSA;  P16856, VNFK_AZOVI;  Q57302, VNFK_TRIV2;  

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