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ENZYME

ENZYME entry: EC 1.18.6.1

PURL: https://purl.expasy.org/enzyme/EC/1.18.6.1
Accepted Name
nitrogenase
Reaction catalysed
N2 + 8 reduced [2Fe-2S]-[ferredoxin] + 16 ATP + 16 H2O = H2 + 8 oxidized [2Fe-2S]-[ferredoxin] + 2 NH4(+) + 16 ADP + 16 phosphate + 6 H(+)
Comment(s)
  • The enzyme is a complex of two components (namely dinitrogen reducatse and dinitrogenase).
  • Dinitrogen reductase is a [4Fe-4S] protein, which, in the presence of two molecules of ATP, transfers an electron from ferredoxin to the dinitrogenase component.
  • Dinitrogenase is a molybdenum-iron protein that reduces dinitrogen to two molecules of ammonia in three successive two-electron reductions via diazene and hydrazine.
  • The reduction is initiated by formation of hydrogen in stoichiometric amounts.
  • Acetylene is reduced to ethylene (but only very slowly to ethane), azide to nitrogen and ammonia, and cyanide to methane and ammonia.
  • In the absence of a suitable substrate, hydrogen is slowly formed.
  • Ferredoxin may be replaced by flavodoxin (see EC 1.19.6.1).
  • The enzyme does not reduce CO (cf. EC 1.18.6.2).
  • Formerly EC 1.18.2.1.
Cross-references
BRENDA1.18.6.1
EC2PDB1.18.6.1
ExplorEnz1.18.6.1
KEGG Ligand Database for Enzyme Nomenclature1.18.6.1
IUBMB Enzyme Nomenclature1.18.6.1
MEDLINEFind literature relating to 1.18.6.1
MetaCyc1.18.6.1
Rhea expert-curated reactions1.18.6.1
UniProtKB/Swiss-Prot
P16266, ANFD_AZOVIO68955, ANFD_HELGEQ07933, ANFD_RHOCA
Q46082, ANFD_RUMHUO68946, ANFG_AZOMAO68943, ANFG_AZOPA
P16268, ANFG_AZOVIQ46244, ANFG_CLOPAQ07934, ANFG_RHOCA
O68940, ANFG_RHORUQ46083, ANFG_RUMHUP16267, ANFK_AZOVI
Q07935, ANFK_RHOCAQ46084, ANFK_RUMHUP06662, NIFD_ACIFI
Q44045, NIFD_ALCFAP33177, NIFD_ANASLP25313, NIFD_AZOBR
P07328, NIFD_AZOVIP06121, NIFD_BRADUP06120, NIFD_BRASP
P00467, NIFD_CLOPAO07642, NIFD_CROS5Q02452, NIFD_FRAAL
P77874, NIFD_HERSEP00466, NIFD_KLEPNQ00239, NIFD_LEPBY
P55170, NIFD_METBAP51605, NIFD_METIVP0CW50, NIFD_METMI
P0CW51, NIFD_METMPO27605, NIFD_METTHP20620, NIFD_METTL
Q50788, NIFD_METTMP52337, NIFD_NOSCOP00464, NIFD_NOSS1
P06769, NIFD_RHICPP00465, NIFD_RHILTP08717, NIFD_RHOCA
Q55029, NIFD_RIPO1P19066, NIFD_SINFNP26251, NIFH1_AZOC5
P26248, NIFH1_AZOCHP00459, NIFH1_AZOVIP00456, NIFH1_CLOPA
Q47917, NIFH1_MASLAP54799, NIFH1_METBAP51602, NIFH1_METIV
O27602, NIFH1_METTHP25767, NIFH1_METTLQ50785, NIFH1_METTM
P00457, NIFH1_NOSS1Q9AKT8, NIFH1_PAEDUP0CY95, NIFH1_RHOCA
D5ANI3, NIFH1_RHOCBP0A3S1, NIFH1_TRIV2P26252, NIFH2_AZOC5
P06118, NIFH2_AZOCHP15335, NIFH2_AZOVIP09552, NIFH2_CLOPA
Q47921, NIFH2_MASLAP54800, NIFH2_METBAP08624, NIFH2_METIV
O26739, NIFH2_METTHP08625, NIFH2_METTLO30577, NIFH2_NOSS1
Q9AKT4, NIFH2_PAEDUQ07942, NIFH2_RHOCAQ44484, NIFH2_TRIV2
P16269, NIFH3_AZOVIP09553, NIFH3_CLOPAP22548, NIFH4_CLOPA
P09554, NIFH5_CLOPAP09555, NIFH6_CLOPAB7JA99, NIFH_ACIF2
B5ER76, NIFH_ACIF5P06661, NIFH_ACIFRQ44044, NIFH_ALCFA
A6TTY3, NIFH_ALKMQP33178, NIFH_ANASLP17303, NIFH_AZOBR
B6YRI6, NIFH_AZOPCP06117, NIFH_BRADUP00463, NIFH_BRASP
A3PLS9, NIFH_CERS1Q3J0H1, NIFH_CERS4O31183, NIFH_CERSP
Q3AR70, NIFH_CHLCHB3EH88, NIFH_CHLL2Q3B2P6, NIFH_CHLL3
B3QQ12, NIFH_CHLP8B3EL81, NIFH_CHLPBA1BEH0, NIFH_CHLPD
A4SFU6, NIFH_CHLPMQ8KC92, NIFH_CHLTEQ97ME5, NIFH_CLOAB
O07641, NIFH_CROS5B8HWE3, NIFH_CYAP4Q47G67, NIFH_DECAR
Q3Z7C7, NIFH_DEHM1C0QKK8, NIFH_DESAHB8FAC4, NIFH_DESAL
B1I0Y8, NIFH_DESAPQ09158, NIFH_DESMCA4J8C2, NIFH_DESRM
P26249, NIFH_ENTAGP08925, NIFH_FRAALQ2J4F8, NIFH_FRACC
Q47922, NIFH_FRASEP46034, NIFH_FRASPB7KG76, NIFH_GLOC7
Q9ZIE4, NIFH_GLUDAP77873, NIFH_HERSEP00458, NIFH_KLEPN
B5XPH2, NIFH_KLEV3Q00240, NIFH_LEPBYA0L6X0, NIFH_MAGMM
C6BX34, NIFH_MARSDP71156, NIFH_MEGGAQ0W443, NIFH_METAR
Q2FUB7, NIFH_METHJQ58289, NIFH_METJAQ8PYY0, NIFH_METMA
P0CW56, NIFH_METMIA3CWW3, NIFH_METMJP0CW57, NIFH_METMP
B8ITG7, NIFH_METNOB0UAK2, NIFH_METS4P06119, NIFH_METVO
P26250, NIFH_NOSCOQ51296, NIFH_NOSS6P52336, NIFH_NOSSN
A8L2C4, NIFH_PARS2Q6D2Y8, NIFH_PECASB4SC59, NIFH_PELPB
B4S9H5, NIFH_PROA2P00462, NIFH_RHIETP20623, NIFH_RHILE
Q98AP7, NIFH_RHILOP00461, NIFH_RHILTP00460, NIFH_RHIME
Q2J1I1, NIFH_RHOP2Q13C78, NIFH_RHOPSP22921, NIFH_RHORU
Q55028, NIFH_RIPO1A7NR80, NIFH_ROSCSA5USK5, NIFH_ROSS1
Q59270, NIFH_RUMCEP19068, NIFH_SINFNC4XRK2, NIFH_SOLM1
A4VJ70, NIFH_STUS1A0LH11, NIFH_SYNFMQ2JTL7, NIFH_SYNJA
Q2JP78, NIFH_SYNJBC5BTB0, NIFH_TERTTC4LAS5, NIFH_TOLAT
P0A3S2, NIFH_TRIAZO34106, NIFH_TRIEIP26254, NIFH_TRITH
Q7M8U8, NIFH_WOLSUQ5NLG3, NIFH_ZYMMOP15052, NIFK_ACIFI
P25314, NIFK_AZOBRP07329, NIFK_AZOVIP20621, NIFK_BRADU
P06122, NIFK_BRASPP11347, NIFK_CLOPAO07643, NIFK_CROS5
Q57118, NIFK_FRAALP77875, NIFK_HERSEP09772, NIFK_KLEPN
P51754, NIFK_METBAP0CW52, NIFK_METMIP0CW53, NIFK_METMP
O27606, NIFK_METTHP20622, NIFK_METTLP95296, NIFK_METTM
P00468, NIFK_NOSS1Q55030, NIFK_RIPO1P19067, NIFK_SINFN
P15332, VNFD_AZOCHO68953, VNFD_AZOPAO68954, VNFD_AZOSA
P16855, VNFD_AZOVIP15333, VNFG_AZOCHO68951, VNFG_AZOSA
P16857, VNFG_AZOVIP15334, VNFK_AZOCHO68949, VNFK_AZOPA
O68952, VNFK_AZOSAP16856, VNFK_AZOVIQ57302, VNFK_TRIV2

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.18.6.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.18.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-