ENZYME

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A new class EC 7, Translocases, has been added to the EC list. It will be part of ENZYME from release 2018_10. Read more about EC 7 here.

ENZYME entry: EC 2.1.1.294

Accepted Name

3-O-phospho-polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol 3-phospho-methyltransferase.

Reaction catalysed

S-adenosyl-L-methionine + 3-O-phospho-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-(alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2))(N)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol <=> S-adenosyl-L-homocysteine + 3-O-methylphospho-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-(alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2))(N)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol

Comment(s)

The enzyme is involved in the biosynthesis of the polymannose O-polysaccharide in the outer leaflet of the membrane of Escherichia coli serotype O9a.
O-polysaccharide structures vary extensively because of differences in the number and type of sugars in the repeat unit.
The dual kinase/methylase WbdD also catalyzes the preceding phosphorylation of alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-(alpha-D- Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man- (1->2))(N)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)- alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol (cf. EC 2.7.1.181).

Cross-references

BRENDA

2.1.1.294

EC2PDB

2.1.1.294

ExplorEnz

2.1.1.294

PRIAM enzyme-specific profiles

2.1.1.294

KEGG Ligand Database for Enzyme Nomenclature

2.1.1.294

IUBMB Enzyme Nomenclature