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ENZYME entry: EC 2.1.1.74

Accepted Name
Methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-methyltransferase (NAD(P)H-oxidizing).
Alternative Name(s)
Folate-dependent ribothymidyl synthase.
Folate/FAD-dependent tRNA T54 methyltransferase.
Reaction catalysed
5,10-methylenetetrahydrofolate + uracil(54) in tRNA + NAD(P)H <=> tetrahydrofolate + 5-methyluracil(54) in tRNA + NAD(P)(+)
Comment(s)
  • A flavoprotein (FAD).
  • Up to 25% of the bases in mature tRNA are post-translationally modified or hypermodified.
  • One almost universal post-translational modification is the conversion of U(54) into ribothymidine in the T-Psi-C loop, and this modification is found in most species studied to date.
  • Unlike this enzyme, which uses 5,10-methylenetetrahydrofolate and NAD(P)H to supply the atoms for methylation of U(54), EC 2.1.1.35 uses S-adenosyl-L-methionine.
  • Formerly EC 2.1.2.12.
Cross-references
BRENDA2.1.1.74
EC2PDB2.1.1.74
ExplorEnz2.1.1.74
PRIAM enzyme-specific profiles2.1.1.74
KEGG Ligand Database for Enzyme Nomenclature2.1.1.74
IUBMB Enzyme Nomenclature2.1.1.74
IntEnz2.1.1.74
MEDLINEFind literature relating to 2.1.1.74
MetaCyc2.1.1.74
Rhea expert-curated reactions2.1.1.74
UniProtKB/Swiss-Prot
Q6F1M4, TMFO1_MESFL;  Q2SS13, TMFO1_MYCCT;  Q6MTM6, TMFO1_MYCMS;  
Q6F0T3, TMFO2_MESFL;  Q2SRN2, TMFO2_MYCCT;  Q6MTB4, TMFO2_MYCMS;  
B0C6V8, TRMFO_ACAM1;  A3DCM0, TRMFO_ACET2;  A9NHD0, TRMFO_ACHLI;  
Q8UET6, TRMFO_AGRFC;  Q9KA24, TRMFO_ALKHC;  B8JES4, TRMFO_ANAD2;  
Q2ILE0, TRMFO_ANADE;  A7HDU6, TRMFO_ANADF;  B4UII2, TRMFO_ANASK;  
B7GGC8, TRMFO_ANOFW;  O66913, TRMFO_AQUAE;  A8ID68, TRMFO_AZOC5;  
C3P5N4, TRMFO_BACAA;  C3L795, TRMFO_BACAC;  A0RHK5, TRMFO_BACAH;  
Q81WK3, TRMFO_BACAN;  B7JJB0, TRMFO_BACC0;  Q732N8, TRMFO_BACC1;  
B7IUJ1, TRMFO_BACC2;  C1EP56, TRMFO_BACC3;  B7HDV5, TRMFO_BACC4;  
B7HLG5, TRMFO_BACC7;  A7GRG2, TRMFO_BACCN;  B9IVC1, TRMFO_BACCQ;  
Q819X4, TRMFO_BACCR;  Q636J4, TRMFO_BACCZ;  Q6HEY3, TRMFO_BACHK;  
Q65JN6, TRMFO_BACLD;  A9VT70, TRMFO_BACMK;  A8FD77, TRMFO_BACP2;  
Q5WFP8, TRMFO_BACSK;  P39815, TRMFO_BACSU;  A7Z4N3, TRMFO_BACVZ;  
A1USB2, TRMFO_BARBK;  Q6G3Y9, TRMFO_BARHE;  A9ISB8, TRMFO_BART1;  
Q6MHW5, TRMFO_BDEBA;  B2IGU6, TRMFO_BEII9;  Q89L21, TRMFO_BRADU;  
A5EJU4, TRMFO_BRASB;  A4YV54, TRMFO_BRASO;  C0ZFA9, TRMFO_BREBN;  
Q2YNL7, TRMFO_BRUA2;  A6X1E3, TRMFO_BRUA4;  Q57DL3, TRMFO_BRUAB;  
A9MAR7, TRMFO_BRUC2;  P64232, TRMFO_BRUME;  A5VQ76, TRMFO_BRUO2;  
B0CLM0, TRMFO_BRUSI;  P64233, TRMFO_BRUSU;  B9MM32, TRMFO_CALBD;  
A4XIW8, TRMFO_CALS8;  Q3AB71, TRMFO_CARHZ;  B0T866, TRMFO_CAUSK;  
Q9A566, TRMFO_CAUVC;  Q3J349, TRMFO_CERS4;  A4WRQ2, TRMFO_CERS5;  
Q11HD9, TRMFO_CHESB;  B5Y8G1, TRMFO_COPPD;  B1WR77, TRMFO_CROS5;  
Q1J148, TRMFO_DEIGD;  Q9RXU7, TRMFO_DEIRA;  Q312C5, TRMFO_DESAG;  
B1I258, TRMFO_DESAP;  Q24UF0, TRMFO_DESHY;  Q6ALS7, TRMFO_DESPS;  
A4J5Z8, TRMFO_DESRM;  Q729K0, TRMFO_DESVH;  A1VBW6, TRMFO_DESVV;  
B5YFC6, TRMFO_DICT6;  B8E2F5, TRMFO_DICTD;  A8LK18, TRMFO_DINSH;  
Q834K1, TRMFO_ENTFA;  Q2NAC8, TRMFO_ERYLH;  B1YIB2, TRMFO_EXIS2;  
C4L614, TRMFO_EXISA;  A7HL16, TRMFO_FERNB;  Q8REM9, TRMFO_FUSNN;  
B5EHR5, TRMFO_GEOBB;  B9M5T8, TRMFO_GEODF;  Q39X89, TRMFO_GEOMG;  
Q74A44, TRMFO_GEOSL;  C6E557, TRMFO_GEOSM;  A5G7S3, TRMFO_GEOUR;  
Q7NFC3, TRMFO_GLOVI;  B0TH93, TRMFO_HELMI;  B4U7L4, TRMFO_HYDS0;  
Q0BYJ6, TRMFO_HYPNA;  Q28PE7, TRMFO_JANSC;  Q1IHM1, TRMFO_KORVE;  
Q5FKE1, TRMFO_LACAC;  Q1G9V1, TRMFO_LACDA;  Q04A02, TRMFO_LACDB;  
Q043R6, TRMFO_LACGA;  A8YV48, TRMFO_LACH4;  Q74JJ8, TRMFO_LACJO;  
Q9CG79, TRMFO_LACLA;  A2RKQ0, TRMFO_LACLM;  Q02YZ3, TRMFO_LACLS;  
Q039E2, TRMFO_LACP3;  Q88W23, TRMFO_LACPL;  Q1WU04, TRMFO_LACS1;  
Q38WZ1, TRMFO_LACSS;  Q1MQ25, TRMFO_LAWIP;  Q92C76, TRMFO_LISIN;  
Q720E5, TRMFO_LISMF;  Q8Y7K1, TRMFO_LISMO;  A0AI79, TRMFO_LISW6;  
B1HQJ3, TRMFO_LYSSC;  A0LDC6, TRMFO_MAGMM;  Q2W4D9, TRMFO_MAGSA;  
Q0ANF3, TRMFO_MARMM;  A9VXT4, TRMFO_METEP;  B1ZES7, TRMFO_METPB;  
B1M5H7, TRMFO_METRJ;  B0UI96, TRMFO_METS4;  B0JFX8, TRMFO_MICAN;  
Q2RJP8, TRMFO_MOOTA;  A5IXT6, TRMFO_MYCAP;  Q9S449, TRMFO_MYXXD;  
B2A334, TRMFO_NATTJ;  Q1QMC9, TRMFO_NITHX;  Q3SRR6, TRMFO_NITWN;  
Q8YNJ4, TRMFO_NOSS1;  Q2G718, TRMFO_NOVAD;  A1B9F0, TRMFO_PARDP;  
A7HXL5, TRMFO_PARL1;  Q3A7H9, TRMFO_PELCD;  A1ALD8, TRMFO_PELPD;  
A5D2W5, TRMFO_PELTS;  C0QTE2, TRMFO_PERMH;  B4RDH0, TRMFO_PHEZH;  
A3PDM1, TRMFO_PROM0;  A2C3G4, TRMFO_PROM1;  A8G5I6, TRMFO_PROM2;  
A2C8E1, TRMFO_PROM3;  A9BEK6, TRMFO_PROM4;  A2BXA3, TRMFO_PROM5;  
Q31AA9, TRMFO_PROM9;  Q7VD04, TRMFO_PROMA;  Q7TUW5, TRMFO_PROMM;  
Q7TU75, TRMFO_PROMP;  A2BRU5, TRMFO_PROMS;  Q46K52, TRMFO_PROMT;  
A8F516, TRMFO_PSELT;  Q2K957, TRMFO_RHIEC;  Q1MHL2, TRMFO_RHIL3;  
Q98LE1, TRMFO_RHILO;  Q92Q15, TRMFO_RHIME;  O68141, TRMFO_RHOCB;  
Q2IWI0, TRMFO_RHOP2;  Q07MJ4, TRMFO_RHOP5;  Q6N5Z1, TRMFO_RHOPA;  
Q213W6, TRMFO_RHOPB;  Q136I8, TRMFO_RHOPS;  Q2RTI8, TRMFO_RHORT;  
A3PJ80, TRMFO_RHOS1;  B9KRK8, TRMFO_RHOSK;  Q166D3, TRMFO_ROSDO;  
Q1ATM0, TRMFO_RUBXD;  Q5LST0, TRMFO_RUEPO;  Q1GGU2, TRMFO_RUEST;  
A6U8P9, TRMFO_SINMW;  Q02C58, TRMFO_SOLUE;  A9FRC1, TRMFO_SORC5;  
Q1GTZ7, TRMFO_SPHAL;  A5V4M8, TRMFO_SPHWW;  A7X1M6, TRMFO_STAA1;  
A6U169, TRMFO_STAA2;  Q2FHI7, TRMFO_STAA3;  Q2FZ31, TRMFO_STAA8;  
A5ISD5, TRMFO_STAA9;  Q2YXL7, TRMFO_STAAB;  Q5HGI1, TRMFO_STAAC;  
A6QGF1, TRMFO_STAAE;  P64234, TRMFO_STAAM;  P64235, TRMFO_STAAN;  
Q6GHI4, TRMFO_STAAR;  Q6G9W2, TRMFO_STAAS;  A8Z3T1, TRMFO_STAAT;  
P64236, TRMFO_STAAW;  B9DPG3, TRMFO_STACT;  Q5HPU1, TRMFO_STAEQ;  
Q8CPH2, TRMFO_STAES;  Q4L5V3, TRMFO_STAHJ;  Q49X36, TRMFO_STAS1;  
Q3K1B8, TRMFO_STRA1;  Q8E5M6, TRMFO_STRA3;  Q8DZX5, TRMFO_STRA5;  
C0M6C7, TRMFO_STRE4;  A8AXH0, TRMFO_STRGC;  P05428, TRMFO_STRMU;  
Q99ZL9, TRMFO_STRP1;  Q04KY2, TRMFO_STRP2;  P0DB36, TRMFO_STRP3;  
B5E446, TRMFO_STRP4;  Q5XC46, TRMFO_STRP6;  C1C6S2, TRMFO_STRP7;  
Q8P106, TRMFO_STRP8;  Q1JBP0, TRMFO_STRPB;  Q1JLM2, TRMFO_STRPC;  
Q1JGS2, TRMFO_STRPD;  Q1J6J1, TRMFO_STRPF;  A2REF7, TRMFO_STRPG;  
B1IBA6, TRMFO_STRPI;  B8ZP43, TRMFO_STRPJ;  Q48TI1, TRMFO_STRPM;  
Q97R84, TRMFO_STRPN;  P0DB37, TRMFO_STRPQ;  B2IP98, TRMFO_STRPS;  
Q8DQ51, TRMFO_STRR6;  A4W125, TRMFO_STRS2;  C0MFF9, TRMFO_STRS7;  
A3CN32, TRMFO_STRSV;  A4VUS8, TRMFO_STRSY;  Q5M014, TRMFO_STRT1;  
Q5M4M7, TRMFO_STRT2;  Q03KX0, TRMFO_STRTD;  B9DS62, TRMFO_STRU0;  
C1CDT9, TRMFO_STRZJ;  C1CK27, TRMFO_STRZP;  C1CRV5, TRMFO_STRZT;  
B2V9U2, TRMFO_SULSY;  Q67PC6, TRMFO_SYMTH;  Q2LT41, TRMFO_SYNAS;  
Q31NM4, TRMFO_SYNE7;  Q2JRF7, TRMFO_SYNJA;  Q2JQ64, TRMFO_SYNJB;  
B1XK17, TRMFO_SYNP2;  Q5N5J0, TRMFO_SYNP6;  A5GLJ3, TRMFO_SYNPW;  
Q7U7T2, TRMFO_SYNPX;  A5GU85, TRMFO_SYNR3;  Q0IB24, TRMFO_SYNS3;  
Q3AX63, TRMFO_SYNS9;  Q3AIZ7, TRMFO_SYNSC;  Q0AYP4, TRMFO_SYNWW;  
Q55692, TRMFO_SYNY3;  B7IFU6, TRMFO_THEAB;  P59109, TRMFO_THEEB;  
A6LJK8, TRMFO_THEM4;  Q9WZJ3, TRMFO_THEMA;  B9KAP3, TRMFO_THENN;  
A5IJ51, TRMFO_THEP1;  B1L7X2, TRMFO_THESQ;  Q72IQ5, TRMFO_THET2;  
Q5SID2, TRMFO_THET8;  Q3MA89, TRMFO_TRIV2;  A7INE1, TRMFO_XANP2;  
Q5NPP5, TRMFO_ZYMMO;  

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