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ENZYME

ENZYME entry: EC 2.1.1.74

Accepted Name
methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-methyltransferase [NAD(P)H-oxidizing]
Alternative Name(s)
folate/FAD-dependent tRNA T54 methyltransferase
folate-dependent ribothymidyl synthase
Reaction catalysed
  • (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH + uridine(54) in tRNA <=> (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NAD(+)
  • (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH + uridine(54) in tRNA <=> (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NADP(+)
Comment(s)
  • A flavoprotein (FAD).
  • Up to 25% of the bases in mature tRNA are post-translationally modified or hypermodified.
  • One almost universal post-translational modification is the conversion of U(54) into ribothymidine in the TPsiC loop, and this modification is found in most species studied to date.
  • Unlike this enzyme, which uses 5,10-methylenetetrahydrofolate and NAD(P)H to supply the atoms for methylation of U(54), EC 2.1.1.35 uses S-adenosyl-L-methionine.
  • Formerly EC 2.1.2.12.
Cross-references
BRENDA2.1.1.74
EC2PDB2.1.1.74
ExplorEnz2.1.1.74
PRIAM enzyme-specific profiles2.1.1.74
KEGG Ligand Database for Enzyme Nomenclature2.1.1.74
IUBMB Enzyme Nomenclature2.1.1.74
IntEnz2.1.1.74
MEDLINEFind literature relating to 2.1.1.74
MetaCyc2.1.1.74
Rhea expert-curated reactions2.1.1.74
UniProtKB/Swiss-Prot
Q6F1M4, TMFO1_MESFLQ2SS13, TMFO1_MYCCTQ6MTM6, TMFO1_MYCMS
Q6F0T3, TMFO2_MESFLQ2SRN2, TMFO2_MYCCTQ6MTB4, TMFO2_MYCMS
B0C6V8, TRMFO_ACAM1A3DCM0, TRMFO_ACET2A9NHD0, TRMFO_ACHLI
Q8UET6, TRMFO_AGRFCB8JES4, TRMFO_ANAD2Q2ILE0, TRMFO_ANADE
A7HDU6, TRMFO_ANADFB4UII2, TRMFO_ANASKB7GGC8, TRMFO_ANOFW
O66913, TRMFO_AQUAEA8ID68, TRMFO_AZOC5C3P5N4, TRMFO_BACAA
C3L795, TRMFO_BACACA0RHK5, TRMFO_BACAHQ81WK3, TRMFO_BACAN
B7JJB0, TRMFO_BACC0Q732N8, TRMFO_BACC1B7IUJ1, TRMFO_BACC2
C1EP56, TRMFO_BACC3B7HDV5, TRMFO_BACC4B7HLG5, TRMFO_BACC7
A7GRG2, TRMFO_BACCNB9IVC1, TRMFO_BACCQQ819X4, TRMFO_BACCR
Q636J4, TRMFO_BACCZQ6HEY3, TRMFO_BACHKQ65JN6, TRMFO_BACLD
A9VT70, TRMFO_BACMKA8FD77, TRMFO_BACP2P39815, TRMFO_BACSU
A7Z4N3, TRMFO_BACVZA1USB2, TRMFO_BARBKQ6G3Y9, TRMFO_BARHE
A9ISB8, TRMFO_BART1Q6MHW5, TRMFO_BDEBAB2IGU6, TRMFO_BEII9
Q89L21, TRMFO_BRADUA5EJU4, TRMFO_BRASBA4YV54, TRMFO_BRASO
C0ZFA9, TRMFO_BREBNQ2YNL7, TRMFO_BRUA2A6X1E3, TRMFO_BRUA4
Q57DL3, TRMFO_BRUABA9MAR7, TRMFO_BRUC2P64232, TRMFO_BRUME
A5VQ76, TRMFO_BRUO2B0CLM0, TRMFO_BRUSIP64233, TRMFO_BRUSU
B9MM32, TRMFO_CALBDA4XIW8, TRMFO_CALS8Q3AB71, TRMFO_CARHZ
B0T866, TRMFO_CAUSKQ9A566, TRMFO_CAUVCA3PJ80, TRMFO_CERS1
Q3J349, TRMFO_CERS4A4WRQ2, TRMFO_CERS5B9KRK8, TRMFO_CERSK
Q11HD9, TRMFO_CHESBB5EHR5, TRMFO_CITBBB5Y8G1, TRMFO_COPPD
B1WR77, TRMFO_CROS5Q1J148, TRMFO_DEIGDQ9RXU7, TRMFO_DEIRA
B1I258, TRMFO_DESAPQ24UF0, TRMFO_DESHYQ6ALS7, TRMFO_DESPS
A4J5Z8, TRMFO_DESRMB5YFC6, TRMFO_DICT6B8E2F5, TRMFO_DICTD
A8LK18, TRMFO_DINSHQ834K1, TRMFO_ENTFAQ2NAC8, TRMFO_ERYLH
B1YIB2, TRMFO_EXIS2C4L614, TRMFO_EXISAA7HL16, TRMFO_FERNB
Q8REM9, TRMFO_FUSNNB9M5T8, TRMFO_GEODFQ39X89, TRMFO_GEOMG
Q74A44, TRMFO_GEOSLC6E557, TRMFO_GEOSMA5G7S3, TRMFO_GEOUR
Q7NFC3, TRMFO_GLOVIQ9KA24, TRMFO_HALH5B0TH93, TRMFO_HELMI
B4U7L4, TRMFO_HYDS0Q0BYJ6, TRMFO_HYPNAQ28PE7, TRMFO_JANSC
Q1IHM1, TRMFO_KORVEQ5FKE1, TRMFO_LACACQ1G9V1, TRMFO_LACDA
Q04A02, TRMFO_LACDBQ043R6, TRMFO_LACGAA8YV48, TRMFO_LACH4
Q74JJ8, TRMFO_LACJOQ9CG79, TRMFO_LACLAA2RKQ0, TRMFO_LACLM
Q02YZ3, TRMFO_LACLSQ039E2, TRMFO_LACP3Q88W23, TRMFO_LACPL
Q38WZ1, TRMFO_LATSSQ1MQ25, TRMFO_LAWIPQ1WU04, TRMFO_LIGS1
Q92C76, TRMFO_LISINQ720E5, TRMFO_LISMFQ8Y7K1, TRMFO_LISMO
A0AI79, TRMFO_LISW6B1HQJ3, TRMFO_LYSSCA0LDC6, TRMFO_MAGMM
Q0ANF3, TRMFO_MARMMA9VXT4, TRMFO_METEPB1ZES7, TRMFO_METPB
B1M5H7, TRMFO_METRJB0UI96, TRMFO_METS4B0JFX8, TRMFO_MICAN
Q2RJP8, TRMFO_MOOTAA5IXT6, TRMFO_MYCAPQ9S449, TRMFO_MYXXD
B2A334, TRMFO_NATTJQ1QMC9, TRMFO_NITHXQ729K0, TRMFO_NITV2
A1VBW6, TRMFO_NITV4Q3SRR6, TRMFO_NITWNQ8YNJ4, TRMFO_NOSS1
Q2G718, TRMFO_NOVADQ312C5, TRMFO_OLEA2A1B9F0, TRMFO_PARDP
A7HXL5, TRMFO_PARL1Q2W4D9, TRMFO_PARM1Q7U7T2, TRMFO_PARMW
A1ALD8, TRMFO_PELPDA5D2W5, TRMFO_PELTSC0QTE2, TRMFO_PERMH
B4RDH0, TRMFO_PHEZHB1XK17, TRMFO_PICP2A3PDM1, TRMFO_PROM0
A2C3G4, TRMFO_PROM1A8G5I6, TRMFO_PROM2A2C8E1, TRMFO_PROM3
A9BEK6, TRMFO_PROM4A2BXA3, TRMFO_PROM5Q31AA9, TRMFO_PROM9
Q7VD04, TRMFO_PROMAQ7TUW5, TRMFO_PROMMQ7TU75, TRMFO_PROMP
A2BRU5, TRMFO_PROMSQ46K52, TRMFO_PROMTA8F516, TRMFO_PSELT
Q2K957, TRMFO_RHIECQ1MHL2, TRMFO_RHIJ3Q98LE1, TRMFO_RHILO
Q92Q15, TRMFO_RHIMEA5V4M8, TRMFO_RHIWRO68141, TRMFO_RHOCB
Q2IWI0, TRMFO_RHOP2Q07MJ4, TRMFO_RHOP5Q6N5Z1, TRMFO_RHOPA
Q213W6, TRMFO_RHOPBQ136I8, TRMFO_RHOPSQ2RTI8, TRMFO_RHORT
Q166D3, TRMFO_ROSDOQ1ATM0, TRMFO_RUBXDQ5LST0, TRMFO_RUEPO
Q1GGU2, TRMFO_RUESTQ5WFP8, TRMFO_SHOC1A6U8P9, TRMFO_SINMW
Q02C58, TRMFO_SOLUEA9FRC1, TRMFO_SORC5Q1GTZ7, TRMFO_SPHAL
A7X1M6, TRMFO_STAA1A6U169, TRMFO_STAA2Q2FHI7, TRMFO_STAA3
Q2FZ31, TRMFO_STAA8A5ISD5, TRMFO_STAA9Q2YXL7, TRMFO_STAAB
Q5HGI1, TRMFO_STAACA6QGF1, TRMFO_STAAEP64234, TRMFO_STAAM
P64235, TRMFO_STAANQ6GHI4, TRMFO_STAARQ6G9W2, TRMFO_STAAS
A8Z3T1, TRMFO_STAATP64236, TRMFO_STAAWB9DPG3, TRMFO_STACT
Q5HPU1, TRMFO_STAEQQ8CPH2, TRMFO_STAESQ4L5V3, TRMFO_STAHJ
Q49X36, TRMFO_STAS1Q3K1B8, TRMFO_STRA1Q8E5M6, TRMFO_STRA3
Q8DZX5, TRMFO_STRA5C0M6C7, TRMFO_STRE4A8AXH0, TRMFO_STRGC
P05428, TRMFO_STRMUQ99ZL9, TRMFO_STRP1Q04KY2, TRMFO_STRP2
P0DB36, TRMFO_STRP3B5E446, TRMFO_STRP4Q5XC46, TRMFO_STRP6
C1C6S2, TRMFO_STRP7Q8P106, TRMFO_STRP8Q1JBP0, TRMFO_STRPB
Q1JLM2, TRMFO_STRPCQ1JGS2, TRMFO_STRPDQ1J6J1, TRMFO_STRPF
A2REF7, TRMFO_STRPGB1IBA6, TRMFO_STRPIB8ZP43, TRMFO_STRPJ
Q48TI1, TRMFO_STRPMQ97R84, TRMFO_STRPNP0DB37, TRMFO_STRPQ
B2IP98, TRMFO_STRPSQ8DQ51, TRMFO_STRR6A4W125, TRMFO_STRS2
C0MFF9, TRMFO_STRS7A3CN32, TRMFO_STRSVA4VUS8, TRMFO_STRSY
Q5M014, TRMFO_STRT1Q5M4M7, TRMFO_STRT2Q03KX0, TRMFO_STRTD
B9DS62, TRMFO_STRU0C1CDT9, TRMFO_STRZJC1CK27, TRMFO_STRZP
C1CRV5, TRMFO_STRZTB2V9U2, TRMFO_SULSYQ67PC6, TRMFO_SYMTH
Q2LT41, TRMFO_SYNASQ3A7H9, TRMFO_SYNC1Q31NM4, TRMFO_SYNE7
Q2JRF7, TRMFO_SYNJAQ2JQ64, TRMFO_SYNJBQ5N5J0, TRMFO_SYNP6
A5GLJ3, TRMFO_SYNPWA5GU85, TRMFO_SYNR3Q0IB24, TRMFO_SYNS3
Q3AX63, TRMFO_SYNS9Q3AIZ7, TRMFO_SYNSCQ0AYP4, TRMFO_SYNWW
Q55692, TRMFO_SYNY3B7IFU6, TRMFO_THEABA6LJK8, TRMFO_THEM4
Q9WZJ3, TRMFO_THEMAB9KAP3, TRMFO_THENNA5IJ51, TRMFO_THEP1
B1L7X2, TRMFO_THESQQ72IQ5, TRMFO_THET2Q5SID2, TRMFO_THET8
P59109, TRMFO_THEVBQ3MA89, TRMFO_TRIV2A7INE1, TRMFO_XANP2
Q5NPP5, TRMFO_ZYMMO

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.1.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.1.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.-.-.-