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ENZYME

ENZYME entry: EC 3.1.1.77

Accepted Name
acyloxyacyl hydrolase
Reaction catalysed
a 3-(acyloxy)acyl derivative of bacterial toxin + H2O = a 3-hydroxyacyl derivative of bacterial toxin + a fatty acid + H(+)
Comment(s)
  • The substrate is lipid A on the reducing end of the toxic lipopolysaccharide (LPS) of Salmonella typhimurium and related organisms.
  • It consists of diglucosamine, beta-D-GlcN-(1->6)-D-GlcN, attached by glycosylation on O-6 of its non-reducing residue, phosphorylated on O-4 of this residue and on O-1 of its potentially reducing residue.
  • Both residues carry 3-(acyloxy)acyl groups on N-2 and O-3.
  • The enzyme from human leukocytes detoxifies the lipid by hydrolyzing the secondary acyl groups from O-3 of the 3-hydroxyacyl groups on the disaccharide (LPS).
  • It also possesses a wide range of phospholipase and acyltransferase activities (e.g. EC 3.1.1.4, EC 3.1.1.5, EC 3.1.1.32 and EC 3.1.1.52), hydrolyzing diacylglycerol and phosphatidyl compounds, but not triacylglycerols.
  • It has a preference for saturated C12-C16 acyl groups.
Cross-references
BRENDA3.1.1.77
EC2PDB3.1.1.77
ExplorEnz3.1.1.77
KEGG Ligand Database for Enzyme Nomenclature3.1.1.77
IUBMB Enzyme Nomenclature3.1.1.77
MEDLINEFind literature relating to 3.1.1.77
MetaCyc3.1.1.77
Rhea expert-curated reactions3.1.1.77
UniProtKB/Swiss-Prot
P28039, AOAH_HUMANO35298, AOAH_MOUSEO18823, AOAH_RABIT
Q7WD07, PAGL_BORBRQ9HVD1, PAGL_PSEAE

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