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ENZYME

ENZYME entry: EC 3.2.1.210

PURL: https://purl.expasy.org/enzyme/EC/3.2.1.210
Accepted Name
endoplasmic reticulum Man8GlcNAc2 1,2-alpha-mannosidase
Reaction catalysed
N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + H2O = N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 7A1,2,3B3) + beta-D-mannose
Comment(s)
  • In yeast this activity is catalyzed by a dedicated enzyme that processes unfolded protein-bound Man8GlcNAc2 N-glycans within the endoplasmic reticulum to Man7GlcNAc2.
  • The exposed alpha-1,6-linked mannose residue in the product enables the recognition by the YOS9 lectin, targeting the proteins for degradation.
  • In mammalian cells this activity is part of the regular processing of N-glycosylated proteins, and is not associated with protein degradation.
  • It is carried out by EC 3.2.1.113.
  • The names of the isomers listed here are based on a nomenclature system proposed by Prien et al.
Cross-references
BRENDA3.2.1.210
EC2PDB3.2.1.210
ExplorEnz3.2.1.210
KEGG Ligand Database for Enzyme Nomenclature3.2.1.210
IUBMB Enzyme Nomenclature3.2.1.210
MEDLINEFind literature relating to 3.2.1.210
MetaCyc3.2.1.210
Rhea expert-curated reactions3.2.1.210

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.2.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.2.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.-.-.-