ENZYME entry: EC 4.1.1.89

Accepted Name
Biotin-dependent malonate decarboxylase.
Alternative Name(s)
Malonate carboxy-lyase (biotin-dependent).
Malonate decarboxylase.
Reaction catalysed
Malonate + H(+) <=> acetate + CO(2)
Comment(s)
  • Two types of malonate decarboxylase are currently known, both of which form multienzyme complexes.
  • The enzyme described here is a biotin-dependent, Na(+)-translocating enzyme that includes both soluble and membrane-bound components.
  • The other type is a biotin-independent cytosolic protein (cf. EC 4.1.1.88).
  • As free malonate is chemically rather inert, it has to be activated prior to decarboxylation.
  • Both enzymes achieve this by exchanging malonate with an acetyl group bound to an acyl-carrier protiein (ACP), to form malonyl-ACP and acetate, with subsequent decarboxylation regenerating the acetyl- bound form of the enzyme.
  • The ACP subunit of both enzymes differs from that found in fatty-acid biosynthesis by having phosphopantethine attached to a serine side- chain as 2'-(5-triphosphoribosyl)-3'-dephospho-CoA rather than as phosphopantetheine 4'-phosphate.
  • In the anaerobic bacterium Malonomonas rubra, the components of the multienzyme complex/enzymes involved in carrying out the reactions of this enzyme are as follows: MadA (EC 2.3.1.187), MadB (EC 4.3.99.2), MadC/MadD (EC 2.1.3.10) and MadH (EC 6.2.1.35).
  • Two other components that are involved are MadE, the acyl-carrier protein and MadF, the biotin protein.
  • The carboxy group is lost with retention of configuration.
Cross-references
PROSITEPDOC50980
BRENDA4.1.1.89
EC2PDB4.1.1.89
ExplorEnz4.1.1.89
PRIAM enzyme-specific profiles4.1.1.89
KEGG Ligand Database for Enzyme Nomenclature4.1.1.89
IUBMB Enzyme Nomenclature4.1.1.89
IntEnz4.1.1.89
MEDLINEFind literature relating to 4.1.1.89
MetaCyc4.1.1.89

View entry in original ENZYME format
View entry in raw text format (no links)
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.1.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.1.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.-.-.-