A new class EC 7, Translocases, has been added to the EC list. It will be part of ENZYME from release 2018_10. Read more about EC 7 here.
ENZYME entry: EC 4.1.99.13
Accepted Name |
(6-4)DNA photolyase.
|
Alternative Name(s) |
DNA photolyase. |
Reaction catalysed |
(6-4) photoproduct (in DNA) <=> 2 pyrimidine residues (in DNA) |
Cofactor(s) |
FAD.
|
Comment(s) |
- The overall repair reaction consists of two distinct steps, one of
which is light-independent and the other one light-dependent.
- In the initial light-independent step, a 6-iminium ion is thought to
be generated via proton transfer induced by two histidines highly
conserved among the (6-4) photolyases.
- This intermediate spontaneously rearranges to form an oxetane
intermediate by intramolecular nucleophilic attack.
- In the subsequent light-driven reaction, one electron is believed to
be transferred from the fully reduced FAD cofactor (FADH(-)) to the
oxetane intermediate thus forming a neutral FADH radical and an
anionic oxetane radical, which spontaneously fractures.
- The excess electron is then back-transferred to the flavin radical
restoring the fully reduced flavin cofactor and a pair of pyrimidine
bases.
|
Cross-references |
PROSITE | PDOC00331 |
BRENDA | 4.1.99.13 |
EC2PDB | 4.1.99.13 |
ExplorEnz | 4.1.99.13 |
PRIAM enzyme-specific profiles | 4.1.99.13 |
KEGG Ligand Database for Enzyme Nomenclature | 4.1.99.13 |
IUBMB Enzyme Nomenclature | 4.1.99.13 |
IntEnz | 4.1.99.13 |
MEDLINE | Find literature relating to 4.1.99.13 |
MetaCyc | 4.1.99.13 |
UniProtKB/Swiss-Prot |
|
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