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ENZYME

ENZYME entry: EC 4.6.1.19

Accepted Name
ribonuclease T2
Alternative Name(s)
ribonuclease II
Reaction catalysed
a ribonucleotidyl-ribonucleotide-RNA + H2O <=> a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + H(+)
Comment(s)
  • A widely distributed family of related enzymes found in protozoans, plants, bacteria, animals and viruses that cleave ssRNA 3'-phosphate group with little base specificity.
  • The enzyme catalyzes a two-stage endonucleolytic cleavage.
  • The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending with a 2',3'-cyclic phosphodiester, which are released from the enzyme.
  • The enzyme then hydrolyzes the cyclic products in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised.
  • The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose.
  • The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
  • Formerly EC 2.7.7.17, EC 3.1.4.23 and EC 3.1.27.1.
Cross-references
BRENDA4.6.1.19
EC2PDB4.6.1.19
ExplorEnz4.6.1.19
PRIAM enzyme-specific profiles4.6.1.19
KEGG Ligand Database for Enzyme Nomenclature4.6.1.19
IUBMB Enzyme Nomenclature4.6.1.19
IntEnz4.6.1.19
MEDLINEFind literature relating to 4.6.1.19
MetaCyc4.6.1.19
Rhea expert-curated reactions4.6.1.19
UniProtKB/Swiss-Prot
Q7M438, RNDI_DICDIP81296, RNL2_LENEDP80022, RNLE_SOLLC
P80196, RNLX_SOLLCP23540, RNMC1_MOMCHP19791, RNM_ASPPH
P08056, RNRH_RHINIQ7SID5, RNS11_NICALP42813, RNS1_ARATH
O80322, RNS1_PYRPYQ38716, RNS2_ANTHIP42814, RNS2_ARATH
P04007, RNS2_NICALQ40965, RNS2_PYRPYQ01796, RNS2_SOLTU
P42815, RNS3_ARATHQ40875, RNS3_PETHYO80323, RNS3_PYRPY
Q38717, RNS4_ANTHIQ40966, RNS4_PYRPYP93460, RNS5_PYRPY
Q40379, RNS6_NICALO80324, RNS6_PYRPYQ40381, RNS7_NICAL
O80325, RNS7_PYRPYO61887, RNST2_CAEELC0HKG5, RNT2A_MOUSE
C0HKG6, RNT2B_MOUSEP10281, RNT2_ASPORB8XY56, RNT2_DANRE
O00584, RNT2_HUMANQ7M329, RNT2_PIGP24657, RNTR_HYPRU
Q5AK94, RNY1A_CANALQ5AKB1, RNY1B_CANALQ75BW5, RNY1_ASHGO
Q4WXZ5, RNY1_ASPFUQ6FP42, RNY1_CANGAQ6BHB1, RNY1_DEBHA
Q5B3K6, RNY1_EMENIQ6CRT6, RNY1_KLULAQ6CAV7, RNY1_YARLI
Q02933, RNY1_YEAST

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.6.1.-
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