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ENZYME entry: EC 4.6.1.19

Accepted Name
Ribonuclease T(2).
Alternative Name(s)
(RNA) 5'-hydroxy-ribonucleotide-3'-(RNA fragment)-lyase (cyclicizing; (RNA fragment)-3'- nucleoside-2',3'-cyclophosphate-forming and hydrolyzing).
Ribonuclease II.
Ribonuclease T2.
Reaction catalysed
RNA + H(2)O <=> an (RNA fragment)-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Comment(s)
  • A widely distributed family of related enzymes found in protozoans, plants, bacteria, animals and viruses that cleave ssRNA 3'-phosphate group with little base specificity.
  • The enzyme catalyzes a two-stage endonucleolytic cleavage.
  • The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending with a 2',3'-cyclic phosphodiester, which are released from the enzyme.
  • The enzyme then hydrolyzes the cyclic products in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised.
  • The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose.
  • The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
  • Formerly EC 2.7.7.17, EC 3.1.4.23, and EC 3.1.27.1.
Cross-references
PROSITEPDOC00459
BRENDA4.6.1.19
EC2PDB4.6.1.19
ExplorEnz4.6.1.19
PRIAM enzyme-specific profiles4.6.1.19
KEGG Ligand Database for Enzyme Nomenclature4.6.1.19
IUBMB Enzyme Nomenclature4.6.1.19
IntEnz4.6.1.19
MEDLINEFind literature relating to 4.6.1.19
MetaCyc4.6.1.19
UniProtKB/Swiss-Prot
Q7M438, RNDI_DICDI;  P81296, RNL2_LENED;  P80022, RNLE_SOLLC;  
P80196, RNLX_SOLLC;  P23540, RNMC_MOMCH;  P19791, RNM_ASPPH;  
P08056, RNRH_RHINI;  Q7SID5, RNS11_NICAL;  P42813, RNS1_ARATH;  
O80322, RNS1_PYRPY;  Q38716, RNS2_ANTHI;  P42814, RNS2_ARATH;  
P04007, RNS2_NICAL;  Q40965, RNS2_PYRPY;  Q01796, RNS2_SOLTU;  
P42815, RNS3_ARATH;  Q40875, RNS3_PETHY;  O80323, RNS3_PYRPY;  
Q38717, RNS4_ANTHI;  Q40966, RNS4_PYRPY;  P93460, RNS5_PYRPY;  
Q40379, RNS6_NICAL;  O80324, RNS6_PYRPY;  Q40381, RNS7_NICAL;  
O80325, RNS7_PYRPY;  O61887, RNST2_CAEEL;  C0HKG5, RNT2A_MOUSE;  
C0HKG6, RNT2B_MOUSE;  P10281, RNT2_ASPOR;  B8XY56, RNT2_DANRE;  
O00584, RNT2_HUMAN;  Q7M329, RNT2_PIG;  P24657, RNTR_HYPRU;  
Q5AK94, RNY1A_CANAL;  Q5AKB1, RNY1B_CANAL;  Q75BW5, RNY1_ASHGO;  
Q4WXZ5, RNY1_ASPFU;  Q6FP42, RNY1_CANGA;  Q6BHB1, RNY1_DEBHA;  
Q5B3K6, RNY1_EMENI;  Q6CRT6, RNY1_KLULA;  Q6CAV7, RNY1_YARLI;  
Q02933, RNY1_YEAST;  

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