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ENZYME entry: EC 4.6.1.24

Accepted Name
Ribonuclease T(1).
Alternative Name(s)
(RNA)-guanosine 5'-hydroxy-ribonucleotide-3'-(RNA fragment)-lyase (cyclicizing; (RNA fragment)-3'-guanosine-2',3'-cyclophosphate-forming and hydrolyzing).
Aspergillus oryzae ribonuclease.
Guanyloribonuclease.
Ribonuclease T1.
RNase N(1).
RNase N(2).
Reaction catalysed
(RNA) containing guanosine + H(2)O <=> an (RNA fragment)-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Comment(s)
  • A family of related enzymes found in some fungi and bacteria.
  • The enzyme is specific for cleavage at the 3'-phosphate group of guanosine in single stranded RNA, and catalyzes a two-stage endonucleolytic cleavage.
  • The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphodiester, which are released from the enzyme.
  • The enzyme then hydrolyzes these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised.
  • The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose.
  • The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
  • Formerly EC 2.7.7.26, EC 3.1.4.8 and EC 3.1.27.3.
Cross-references
BRENDA4.6.1.24
EC2PDB4.6.1.24
ExplorEnz4.6.1.24
PRIAM enzyme-specific profiles4.6.1.24
KEGG Ligand Database for Enzyme Nomenclature4.6.1.24
IUBMB Enzyme Nomenclature4.6.1.24
IntEnz4.6.1.24
MEDLINEFind literature relating to 4.6.1.24
MetaCyc4.6.1.24
UniProtKB/Swiss-Prot
Q9TZQ3, PGL1_CAEEL;  E3M3V1, PGL1_CAERE;  G5EBV6, PGL3_CAEEL;  
Q7M515, RNA1_ASPPL;  P00652, RNC2_ASPCL;  D4AVI0, RNF1_ARTBC;  
P16411, RNF1_GIBBA;  P10282, RNF1_GIBFU;  P16412, RNF2_GIBBA;  
P00653, RNMS_ASPPH;  P09646, RNN1_NEUCR;  P07446, RNPB_PENBR;  
P09647, RNPC_PENCH;  P81762, RNPO_PLEOS;  P30289, RNS3_KITAU;  
P05798, RNSA_KITAU;  P00650, RNST_SACER;  P00651, RNT1_ASPOR;  
P26875, RNT1_TRIHA;  P07736, RNU1_USTSP;  

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