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ENZYME

ENZYME entry: EC 4.6.1.24

Accepted Name
ribonuclease T1
Alternative Name(s)
Aspergillus oryzae ribonuclease
guanyloribonuclease
RNase N1
RNase N2
Reaction catalysed
[RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
Comment(s)
  • A family of related enzymes found in some fungi and bacteria.
  • The enzyme is specific for cleavage at the 3'-phosphate group of guanosine in single stranded RNA, and catalyzes a two-stage endonucleolytic cleavage.
  • The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphodiester, which are released from the enzyme.
  • The enzyme then hydrolyzes these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised.
  • The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose.
  • The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
  • Formerly EC 2.7.7.26, EC 3.1.4.8 and EC 3.1.27.3.
Cross-references
BRENDA4.6.1.24
EC2PDB4.6.1.24
ExplorEnz4.6.1.24
PRIAM enzyme-specific profiles4.6.1.24
KEGG Ligand Database for Enzyme Nomenclature4.6.1.24
IUBMB Enzyme Nomenclature4.6.1.24
IntEnz4.6.1.24
MEDLINEFind literature relating to 4.6.1.24
MetaCyc4.6.1.24
Rhea expert-curated reactions4.6.1.24
UniProtKB/Swiss-Prot
Q9TZQ3, PGL1_CAEELE3M3V1, PGL1_CAEREG5EBV6, PGL3_CAEEL
Q7M515, RNA1_ASPPLP00652, RNC2_ASPCLD4AVI0, RNF1_ARTBC
P16411, RNF1_GIBBAP10282, RNF1_GIBFUP16412, RNF2_GIBBA
P00653, RNMS_ASPPHP09646, RNN1_NEUCRP07446, RNPB_PENBR
P09647, RNPC_PENCHP81762, RNPO_PLEOSP30289, RNS3_KITAU
P05798, RNSA_KITAUP00650, RNST_SACERP00651, RNT1_ASPOR
P26875, RNT1_TRIHAP07736, RNU1_USTSP

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