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ENZYME entry: EC 4.8.1.4

Accepted Name
phenylacetaldoxime dehydratase.
Alternative Name(s)
(Z)-phenylacetaldehyde-oxime hydro-lyase.
arylacetaldoxime dehydratase.
PAOx dehydratase.
Reaction catalysed
(Z)-phenylacetaldehyde oxime <=> H2O + phenylacetonitrile
Comment(s)
  • The enzyme from Bacillus sp. OxB-1 contains protoheme IX, the iron of which must be in the form iron(II) for activity.
  • (Z)-Phenylacetaldoxime binds to ferric heme (the iron(III) form) via the oxygen atom whereas it binds to the active ferrous form via the nitrogen atom.
  • In this way, the oxidation state of the heme controls the coordination stucture of the substrate--heme complex, which regulates enzyme activity.
  • The enzyme is active toward several (Z)-arylacetaldoximes and (E/Z)- alkylaldoximes as well as toward arylalkylaldoximes such as 3-phenylpropionaldoxime and 4-phenylbutyraldoxime.
  • However, it is inactive with phenylacetaldoximes that have a substituent group at an alpha-site of an oxime group, for example, with (E/Z)-2-phenylpropionaldoxime and (E/Z)-mandelaldoxime.
  • The activity of the enzyme is inhibited completely by the heavy-metal cations Cu(+), Cu(2+), Ag(+) and Hg(+) whereas Fe(2+) and Sn(2+) have an activatory effect.
  • Formerly EC 4.99.1.7.
Cross-references
BRENDA4.8.1.4
EC2PDB4.8.1.4
ExplorEnz4.8.1.4
PRIAM enzyme-specific profiles4.8.1.4
KEGG Ligand Database for Enzyme Nomenclature4.8.1.4
IUBMB Enzyme Nomenclature4.8.1.4
IntEnz4.8.1.4
MEDLINEFind literature relating to 4.8.1.4
MetaCyc4.8.1.4
Rhea expert-curated reactions4.8.1.4
UniProtKB/Swiss-Prot
P82604, OXD_BACSX

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