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ENZYME

ENZYME entry: EC 6.2.2.1

PURL: https://purl.expasy.org/enzyme/EC/6.2.2.1
Accepted Name
thioglycine synthase
Reaction catalysed
glycyl-[methyl-coenzyme M reductase] + hydrogen sulfide + ATP = thioglycyl-[methyl-coenzyme M reductase] + ADP + phosphate
Comment(s)
  • Requires Mg(2+).
  • The enzyme is found in anaerobic methanogenic and methanotrophic archaea, where it modifies a glycine residue in EC 2.8.4.1.
  • Upon binding to its substrate, an external source of sulfide attacks the target amide bond generating a tetrahedral intermediate.
  • The amide oxyanion attacks the gamma-phosphate of ATP, releasing ADP and forming a phosphorylated thiolate intermediate that collapses to form thioglycine and phosphate.
  • In most organisms activity requires a second protein (TfuA) , which may allosterically activate this enzyme or assist in the delivery of sulfide to the substrate.
Cross-references
BRENDA6.2.2.1
EC2PDB6.2.2.1
ExplorEnz6.2.2.1
KEGG Ligand Database for Enzyme Nomenclature6.2.2.1
IUBMB Enzyme Nomenclature6.2.2.1
MEDLINEFind literature relating to 6.2.2.1
MetaCyc6.2.2.1
Rhea expert-curated reactions6.2.2.1

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.2.2.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.2.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-