ENZYME

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A new class EC 7, Translocases, has been added to the EC list. It will be part of ENZYME from release 2018_10. Read more about EC 7 here.

ENZYME entry: EC 6.3.1.12

Accepted Name

D-aspartate ligase.

Alternative Name(s)

Asl(fm).

D-aspartic acid-activating enzyme.

Reaction catalysed

ATP + D-aspartate + (beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n) <=> (beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala))(n) + ADP + phosphate

Comment(s)

Forms part of the peptidoglycan assembly pathway of Gram-positive bacteria grown in medium containing D-Asp.
Normally, the side chains the acylate the 6-amino group of the L-lysine residue contain L-Ala-L-Ala but these amino acids are replaced by D-Asp when D-Asp is included in the medium.
Hybrid chains containing L-Ala-D-Asp, L-Ala-L-Ala-D-Asp or D-Asp-L- Ala are not formed.
Highly specific for D-aspartate, as L-aspartate, D-glutamate, D-alanine, D-iso-asparagine and D-malic acid are not substrates.
In Enterococcus faecium, the substrate D-aspartate is produced by EC 5.1.1.13.

Cross-references

PROSITE

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

6.3.1.12

KEGG Ligand Database for Enzyme Nomenclature