ENZYME entry: EC 6.3.1.19

PURL: https://purl.expasy.org/enzyme/EC/6.3.1.19

Accepted Name

prokaryotic ubiquitin-like protein ligase

Alternative Name(s)

proteasome accessory factor A

Pup-conjugating enzyme

Pup ligase

Pup--protein ligase

Reaction catalysed

ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N(6)-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine

Comment(s)

The enzyme has been characterized from the bacteria Mycobacterium tuberculosis and Corynebacterium glutamicum.
It catalyzes the ligation of the prokaryotic ubiquitin-like protein (Pup) to a target protein by forming a bond between an epsilon-amino group of a lysine residue of the target protein and the gamma- carboxylate of the C-terminal glutamate of the ubiquitin-like protein (Pup).
The attachment of Pup, also known as Pupylation, marks proteins for proteasomal degradation.
Formerly EC 6.3.2.n2.

Cross-references

Rhea expert-curated reactions

6.3.1.19

ExplorEnz

6.3.1.19

IUBMB Enzyme Nomenclature

6.3.1.19

UniProtKB/Swiss-Prot

P72263, PAFA1_RHOER	P72270, PAFA2_RHOER	A0LU53, PAFA_ACIC1
C7LYP9, PAFA_ACIFD	C6WIE4, PAFA_ACTMD	A0JWY8, PAFA_ARTS2
B8DTX8, PAFA_BIFA0	A1A0U7, PAFA_BIFAA	C6AHX4, PAFA_BIFAS
D2Q9D0, PAFA_BIFDB	C6A7B6, PAFA_BIFLB	B3DRN8, PAFA_BIFLD
Q8G3G3, PAFA_BIFLO	B7GUN9, PAFA_BIFLS	C7MCY7, PAFA_BRAFD
C7PVV4, PAFA_CATAD	C3PGA3, PAFA_CORA7	Q6NH95, PAFA_CORDI
Q8FTE6, PAFA_COREF	A4QE80, PAFA_CORGB	Q8NQE1, PAFA_CORGL
Q4JVP8, PAFA_CORJK	C4LIK9, PAFA_CORK4	B1VDV5, PAFA_CORU7
Q0RLT2, PAFA_FRAAA	Q2J9R8, PAFA_FRACC	D2S6E4, PAFA_GEOOG
D0LDT3, PAFA_GORB4	C7R405, PAFA_JONDD	A6W972, PAFA_KINRD
B2GIN9, PAFA_KOCRD	D2Q3K3, PAFA_KRIFD	C5CBV0, PAFA_MICLC
A0QFB7, PAFA_MYCA1	B1MAJ3, PAFA_MYCA9	P64944, PAFA_MYCBO
A1KKE3, PAFA_MYCBP	C1AQ16, PAFA_MYCBT	A4TB47, PAFA_MYCGI
B8ZRF5, PAFA_MYCLB	P54077, PAFA_MYCLE	B2HFV4, PAFA_MYCMM
Q73YX0, PAFA_MYCPA	A0QZ42, PAFA_MYCS2	A3PZG1, PAFA_MYCSJ
A1UFV1, PAFA_MYCSK	Q1B959, PAFA_MYCSS	A5U4C3, PAFA_MYCTA
A5WP74, PAFA_MYCTF	C6DPW7, PAFA_MYCTK	P9WNU6, PAFA_MYCTO
P9WNU7, PAFA_MYCTU	A0PQT1, PAFA_MYCUA	A1TAP0, PAFA_MYCVP
C8XAP5, PAFA_NAKMY	Q5YUX4, PAFA_NOCFA	A1SK16, PAFA_NOCSJ
A1R6Q9, PAFA_PAEAT	A8LH49, PAFA_PARS2	B8H8L8, PAFA_PSECP
A9WSH9, PAFA_RENSM	C0ZZU4, PAFA_RHOE4	Q0SIG2, PAFA_RHOJR
C1ASP4, PAFA_RHOOB	D2NT93, PAFA_ROTMD	A4FBY4, PAFA_SACEN
C7MWV3, PAFA_SACVD	A8M2A6, PAFA_SALAI	A4X747, PAFA_SALTO
D1BHT7, PAFA_SANKS	Q828I4, PAFA_STRAW	Q9RJ61, PAFA_STRCO
B1W310, PAFA_STRGG	D2ATU8, PAFA_STRRD	C9Z4D4, PAFA_STRSW
D1A2T1, PAFA_THECD	Q47NZ6, PAFA_THEFY	D1BS28, PAFA_XYLCX

BRENDA

6.3.1.19

MetaCyc

6.3.1.19

KEGG Ligand Database for Enzyme Nomenclature

6.3.1.19

EC2PDB

6.3.1.19

MEDLINE

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.3.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.3.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-