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ENZYME

ENZYME entry: EC 6.5.1.1

Accepted Name
DNA ligase (ATP)
Alternative Name(s)
DNA joinase
DNA repair enzyme
polydeoxyribonucleotide synthase (ATP)
polynucleotide ligase (ATP)
sealase
Reaction catalysed
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m <=> (deoxyribonucleotide)n+m + AMP + diphosphate
Comment(s)
  • The enzyme catalyzes the ligation of DNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in duplex DNA.
  • Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue.
  • The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-[DNA].
  • Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the adenylate.
  • RNA can also act as substrate, to some extent. cf. EC 6.5.1.2, EC 6.5.1.6 and EC 6.5.1.7.
Cross-references
BRENDA6.5.1.1
EC2PDB6.5.1.1
ExplorEnz6.5.1.1
PRIAM enzyme-specific profiles6.5.1.1
KEGG Ligand Database for Enzyme Nomenclature6.5.1.1
IUBMB Enzyme Nomenclature6.5.1.1
IntEnz6.5.1.1
MEDLINEFind literature relating to 6.5.1.1
MetaCyc6.5.1.1
Rhea expert-curated reactions6.5.1.1
UniProtKB/Swiss-Prot
Q42572, DNLI1_ARATHQ27474, DNLI1_CAEELQ869E1, DNLI1_DICDI
Q9W1H4, DNLI1_DROMEQ18HT7, DNLI1_HALWDP18858, DNLI1_HUMAN
B1L3K8, DNLI1_KORCOQ8TSR7, DNLI1_METACQ46C04, DNLI1_METBF
Q8PVR7, DNLI1_METMAP37913, DNLI1_MOUSEQ9JHY8, DNLI1_RAT
P12000, DNLI1_SCHPOA1RXA6, DNLI1_THEPDP51892, DNLI1_XENLA
P04819, DNLI1_YEASTQ18GX5, DNLI2_HALWDB1L3V2, DNLI2_KORCO
Q8TMT1, DNLI2_METACQ46BA3, DNLI2_METBFQ8PTK1, DNLI2_METMA
A1RY72, DNLI2_THEPDQ54QM4, DNLI3_DICDIP49916, DNLI3_HUMAN
P97386, DNLI3_MOUSEQ9C1W9, DNLI3_SCHPOQ9LL84, DNLI4_ARATH
Q75CA4, DNLI4_ASHGOQ4WVG8, DNLI4_ASPFUQ2U6A1, DNLI4_ASPOR
P52496, DNLI4_CANALQ6FVD8, DNLI4_CANGBQ90YB1, DNLI4_CHICK
Q1DKE7, DNLI4_COCIMA8N936, DNLI4_COPC7Q7Z7W5, DNLI4_COPCI
G3GTP0, DNLI4_CRIGRP0CN09, DNLI4_CRYNBP0CN08, DNLI4_CRYNJ
Q54CR9, DNLI4_DICDIQ5BH83, DNLI4_EMENIP49917, DNLI4_HUMAN
Q6CSH0, DNLI4_KLULCQ8BTF7, DNLI4_MOUSEQ7SB49, DNLI4_NEUCR
Q7X7E9, DNLI4_ORYSJQ0UCI9, DNLI4_PHANOQ5R6L3, DNLI4_PONAB
O74833, DNLI4_SCHPOQ6C8A3, DNLI4_YARLIQ08387, DNLI4_YEAST
Q02093, DNLI_ACIAMB8JBM1, DNLI_ANAD2Q2IH69, DNLI_ANADE
A7HID3, DNLI_ANADFB4UIS1, DNLI_ANASKO67398, DNLI_AQUAE
O29632, DNLI_ARCFUP35970, DNLI_ASFB7P0C992, DNLI_ASFK5
P26813, DNLI_ASFM2P0C990, DNLI_ASFP4P0C991, DNLI_ASFWA
C5BW79, DNLI_BEUC1P07717, DNLI_BPT3P00970, DNLI_BPT4
P19088, DNLI_BPT6P00969, DNLI_BPT7A8MDU6, DNLI_CALMQ
A0RWD6, DNLI_CENSYB5YD88, DNLI_DICT6B8DZX8, DNLI_DICTD
Q67480, DNLI_FOWPNP44121, DNLI_HAEINB9LUZ9, DNLI_HALLT
Q5V4R0, DNLI_HALMAB0R4C9, DNLI_HALS3Q9HR35, DNLI_HALSA
D4GYZ4, DNLI_HALVDA8AB20, DNLI_IGNH4A6WG40, DNLI_KINRD
Q1II25, DNLI_KORVEA6UVC4, DNLI_META3Q0W0X1, DNLI_METAR
A7IAA9, DNLI_METB6Q12X08, DNLI_METBUQ2FTH4, DNLI_METHJ
Q57635, DNLI_METJAQ8TWN3, DNLI_METKAA2SR38, DNLI_METLZ
A4FY19, DNLI_METM5A9AAX4, DNLI_METM6A6VFQ9, DNLI_METM7
A3CWP1, DNLI_METMJQ6LYM1, DNLI_METMPB8GGB8, DNLI_METPE
A5UKX2, DNLI_METS3A4YD25, DNLI_METS5Q2NHF9, DNLI_METST
P54875, DNLI_METTFQ50566, DNLI_METTHA0B7F9, DNLI_METTP
A6UNY0, DNLI_METVSA0A7H0DNE6, DNLI_MONPVA0QJL0, DNLI_MYCA1
Q7TTR7, DNLI_MYCBOA1KN60, DNLI_MYCBPC1AGI1, DNLI_MYCBT
A4TDS2, DNLI_MYCGIB2HHE1, DNLI_MYCMMQ73V97, DNLI_MYCPA
A0QUP1, DNLI_MYCS2A3PXE2, DNLI_MYCSJA1UDY9, DNLI_MYCSK
Q1BB26, DNLI_MYCSSA5U776, DNLI_MYCTAP9WNV4, DNLI_MYCTO
P9WNV5, DNLI_MYCTUA0PTC0, DNLI_MYCUAA1T6P2, DNLI_MYCVP
Q74M64, DNLI_NANEQQ3IPX0, DNLI_NATPDA9A3K1, DNLI_NITMS
Q5YNY7, DNLI_NOCFAA1SKL2, DNLI_NOCSJQ9YMV2, DNLI_NPVLD
B1ZZL9, DNLI_OPITPC0QSL7, DNLI_PERMHQ6L195, DNLI_PICTO
B8HCE1, DNLI_PSECPP0CL75, DNLI_PYRABO93723, DNLI_PYRAE
A4WH24, DNLI_PYRARP0CL74, DNLI_PYRAYA3MS62, DNLI_PYRCJ
P56709, DNLI_PYRFUO59288, DNLI_PYRHOA1RTK4, DNLI_PYRIL
B1YA52, DNLI_PYRNVQ0SG75, DNLI_RHOJRC1AX87, DNLI_RHOOB
A4F870, DNLI_SACENQ980T8, DNLI_SACS2Q9P9K9, DNLI_SACSH
A9GU24, DNLI_SORC5Q826P6, DNLI_STRAWQ9FCB1, DNLI_STRCO
B1W5J2, DNLI_STRGGQ4JAM1, DNLI_SULACC3MZR1, DNLI_SULIA
C4KIZ2, DNLI_SULIKC3MJ14, DNLI_SULILC3MYD2, DNLI_SULIM
C3NF77, DNLI_SULINC3N834, DNLI_SULIYQ976G4, DNLI_SULTO
Q9HJ26, DNLI_THEACQ979A1, DNLI_THEVOO57250, DNLI_VACCA
P20492, DNLI_VACCCP16272, DNLI_VACCWP0DOO3, DNLI_VAR67
P0DOO4, DNLI_VARVF4HPZ9, LIG6_ARATHA0R5T2, LIGC1_MYCS2
A0R5T3, LIGC2_MYCS2L0TDE1, LIGC_MYCTUO34398, LIGD_BACSU
P59971, LIGD_MYCBOA0R3R7, LIGD_MYCS2P9WNV2, LIGD_MYCTO
P9WNV3, LIGD_MYCTUQ9I1X7, LIGD_PSEAEO54003, REP75_PYRAB
P03631, REPA_BPPHSP19189, REP_BPCHPP69546, REP_BPF1
P69545, REP_BPFDP15419, REP_BPI22O80302, REP_BPIF1
P03660, REP_BPIKEP69547, REP_BPM13P03627, REP_BPPF3
Q38617, REP_BPPHLQ9G050, REP_BPPHMP11334, REP_SPV4

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.5.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.5.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-