ENZYME entry: EC 22.214.171.124
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|RNA 3'-terminal-phosphate cyclase (GTP).
|RNA-3'-phosphate cyclase (GTP).|
|GTP + (RNA)-3'-(3'-phospho-ribonucleoside) <=> GMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside|
- The enzyme, which is specific for GTP, was characterized from the
archaeon Pyrococcus furiosus.
- The enzyme converts the 3'-terminal phosphate of various RNA
substrates into the 2',3'-cyclic phosphodiester in a GTP-dependent
- Catalysis occurs by a three-step mechanism, starting with the
activation of the enzyme by GTP, forming a phosphoramide bond between
guanylate and a histidine residue.
- The guanylate group is then transferred to the 3'-phosphate terminus
of the substrate, forming the capped structure (RNA)-
- Finally, the enzyme catalyzes an attack of the vicinal O-2' on the
3'-phosphorus, which results in formation of cyclic phosphate and
release of the guanylate.
- Cf. EC 126.96.36.199.
|PRIAM enzyme-specific profiles||188.8.131.52|
|KEGG Ligand Database for Enzyme Nomenclature||184.108.40.206|
|IUBMB Enzyme Nomenclature||220.127.116.11|
|MEDLINE||Find literature relating to 18.104.22.168|
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