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ENZYME

ENZYME entry: EC 6.5.1.5

PURL: https://purl.expasy.org/enzyme/EC/6.5.1.5
Accepted Name
RNA 3'-terminal-phosphate cyclase (GTP)
Alternative Name(s)
RNA-3'-phosphate cyclase (GTP)
Reaction catalysed
a 3'-end 3'-phospho-ribonucleotide-RNA + GTP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + GMP + diphosphate
Comment(s)
  • The enzyme, which is specific for GTP, was characterized from the archaeon Pyrococcus furiosus.
  • The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in a GTP-dependent reaction.
  • Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by GTP, forming a phosphoramide bond between guanylate and a histidine residue.
  • The guanylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]- 3'-(5'-diphosphoguanosine).
  • Finally, the enzyme catalyzes an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the guanylate. cf. EC 6.5.1.4.
Cross-references
BRENDA6.5.1.5
EC2PDB6.5.1.5
ExplorEnz6.5.1.5
KEGG Ligand Database for Enzyme Nomenclature6.5.1.5
IUBMB Enzyme Nomenclature6.5.1.5
MEDLINEFind literature relating to 6.5.1.5
MetaCyc6.5.1.5
Rhea expert-curated reactions6.5.1.5
UniProtKB/Swiss-Prot
Q8U0N7, RTCA_PYRFU

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