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ENZYME

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ENZYME entry: EC 6.5.1.4

Accepted Name
RNA 3'-terminal-phosphate cyclase (ATP)
Alternative Name(s)
RNA-3'-phosphate cyclase
RNA cyclase
Reaction catalysed
a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate
Comment(s)
  • The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in an ATP-dependent reaction.
  • Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a histidine residue.
  • The adenylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]- 3'-(5'-diphosphoadenosine).
  • Finally, the enzyme catalyzes an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the adenylate.
  • The enzyme also has a polynucleotide 5' adenylation activity. cf. EC 6.5.1.5.
Cross-references
BRENDA6.5.1.4
EC2PDB6.5.1.4
ExplorEnz6.5.1.4
PRIAM enzyme-specific profiles6.5.1.4
KEGG Ligand Database for Enzyme Nomenclature6.5.1.4
IUBMB Enzyme Nomenclature6.5.1.4
IntEnz6.5.1.4
MEDLINEFind literature relating to 6.5.1.4
MetaCyc6.5.1.4
Rhea expert-curated reactions6.5.1.4
UniProtKB/Swiss-Prot
Q9YES0, RTCA_AERPEO66884, RTCA_AQUAEO28837, RTCA_ARCFU
Q2HJ88, RTCA_BOVINA8MAV7, RTCA_CALMQB8D680, RTCA_DESA1
O15746, RTCA_DICDIO77264, RTCA_DROMEA7ZSU9, RTCA_ECO24
B7UKC4, RTCA_ECO27B7MDP5, RTCA_ECO45B7L4U9, RTCA_ECO55
P58127, RTCA_ECO57B5YTX2, RTCA_ECO5EB7NMI4, RTCA_ECO7I
B7N1J7, RTCA_ECO81B7M2I0, RTCA_ECO8AC4ZVX1, RTCA_ECOBW
B1X765, RTCA_ECODHA8A5M8, RTCA_ECOHSA1AGU3, RTCA_ECOK1
Q0TC48, RTCA_ECOL5Q8FCS8, RTCA_ECOL6B1IP46, RTCA_ECOLC
P46849, RTCA_ECOLIB7NE24, RTCA_ECOLUB1LI80, RTCA_ECOSM
Q1R5L5, RTCA_ECOUTB7LSC2, RTCA_ESCF3B7KCF3, RTCA_GLOC7
B0R414, RTCA_HALS3Q9HRE8, RTCA_HALSAO00442, RTCA_HUMAN
A2BN21, RTCA_HYPBUA8A9W2, RTCA_IGNH4B1L6E9, RTCA_KORCO
Q1IS76, RTCA_KORVEQ4R3J0, RTCA_MACFAQ8TH85, RTCA_METAC
Q46DV9, RTCA_METBFQ12V70, RTCA_METBUQ609M1, RTCA_METCA
Q60335, RTCA_METJAQ8TZC9, RTCA_METKAQ8PXC4, RTCA_METMA
A4YEC3, RTCA_METS5O27937, RTCA_METTHA0B532, RTCA_METTP
Q9D7H3, RTCA_MOUSEQ2Y720, RTCA_NITMUB2IWJ8, RTCA_NOSP7
Q8YVS3, RTCA_NOSS1Q5R7P3, RTCA_PONABB7V0C8, RTCA_PSEA8
Q02G92, RTCA_PSEABQ9HVJ9, RTCA_PSEAEQ4KEB8, RTCA_PSEF5
A4XRJ0, RTCA_PSEMYQ9V0Z6, RTCA_PYRABQ8ZST4, RTCA_PYRAE
A3MXQ7, RTCA_PYRCJO59198, RTCA_PYRHOA1RSJ7, RTCA_PYRIL
B1YDJ1, RTCA_PYRNVQ8Y2V6, RTCA_RALN1Q97W04, RTCA_SACS2
B5F8N4, RTCA_SALA4B5R378, RTCA_SALEPB5R7J6, RTCA_SALG2
B4TKU5, RTCA_SALHSC0Q0J3, RTCA_SALPCQ8ZLI0, RTCA_SALTY
B2U3M8, RTCA_SHIB3Q31VL3, RTCA_SHIBSQ32AN4, RTCA_SHIDS
Q3YWK6, RTCA_SHISSA3DN24, RTCA_STAMFQ4JB78, RTCA_SULAC
C3N179, RTCA_SULIAC4KKE6, RTCA_SULIKC3MKC1, RTCA_SULIL
C3MU91, RTCA_SULIMC3NMT8, RTCA_SULINC3N969, RTCA_SULIY
Q974U1, RTCA_SULTOC5A2A3, RTCA_THEGJQ5JIQ0, RTCA_THEKO
B6YSJ5, RTCA_THEONC6A4N4, RTCA_THESMQ3M6L1, RTCA_TRIV2

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All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.5.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.5.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-