ENZYME entry: EC 6.5.1.4

PURL: https://purl.expasy.org/enzyme/EC/6.5.1.4

Accepted Name

RNA 3'-terminal-phosphate cyclase (ATP)

Alternative Name(s)

RNA-3'-phosphate cyclase

RNA cyclase

Reaction catalysed

a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate

Comment(s)

The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in an ATP-dependent reaction.
Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a histidine residue.
The adenylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]- 3'-(5'-diphosphoadenosine).
Finally, the enzyme catalyzes an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the adenylate.
The enzyme also has a polynucleotide 5' adenylation activity. cf. EC 6.5.1.5.

Cross-references

BRENDA

6.5.1.4

EC2PDB

6.5.1.4

ExplorEnz

6.5.1.4

KEGG Ligand Database for Enzyme Nomenclature

6.5.1.4

IUBMB Enzyme Nomenclature

6.5.1.4

MEDLINE

Find literature relating to 6.5.1.4

MetaCyc

6.5.1.4

Rhea expert-curated reactions

6.5.1.4

UniProtKB/Swiss-Prot

Q9YES0, RTCA_AERPE	O66884, RTCA_AQUAE	O28837, RTCA_ARCFU
Q2HJ88, RTCA_BOVIN	A8MAV7, RTCA_CALMQ	B8D680, RTCA_DESA1
O15746, RTCA_DICDI	O77264, RTCA_DROME	A7ZSU9, RTCA_ECO24
B7UKC4, RTCA_ECO27	B7MDP5, RTCA_ECO45	B7L4U9, RTCA_ECO55
P58127, RTCA_ECO57	B5YTX2, RTCA_ECO5E	B7NMI4, RTCA_ECO7I
B7N1J7, RTCA_ECO81	B7M2I0, RTCA_ECO8A	C4ZVX1, RTCA_ECOBW
B1X765, RTCA_ECODH	A8A5M8, RTCA_ECOHS	A1AGU3, RTCA_ECOK1
Q0TC48, RTCA_ECOL5	Q8FCS8, RTCA_ECOL6	B1IP46, RTCA_ECOLC
P46849, RTCA_ECOLI	B7NE24, RTCA_ECOLU	B1LI80, RTCA_ECOSM
Q1R5L5, RTCA_ECOUT	A4XRJ0, RTCA_ECTM1	B7LSC2, RTCA_ESCF3
B7KCF3, RTCA_GLOC7	B0R414, RTCA_HALS3	Q9HRE8, RTCA_HALSA
O00442, RTCA_HUMAN	A2BN21, RTCA_HYPBU	A8A9W2, RTCA_IGNH4
B1L6E9, RTCA_KORCO	Q1IS76, RTCA_KORVE	Q4R3J0, RTCA_MACFA
Q8TH85, RTCA_METAC	Q46DV9, RTCA_METBF	Q12V70, RTCA_METBU
Q609M1, RTCA_METCA	Q60335, RTCA_METJA	Q8TZC9, RTCA_METKA
Q8PXC4, RTCA_METMA	A4YEC3, RTCA_METS5	O27937, RTCA_METTH
A0B532, RTCA_METTP	Q9D7H3, RTCA_MOUSE	Q2Y720, RTCA_NITMU
B2IWJ8, RTCA_NOSP7	Q8YVS3, RTCA_NOSS1	Q5R7P3, RTCA_PONAB
B7V0C8, RTCA_PSEA8	Q02G92, RTCA_PSEAB	Q9HVJ9, RTCA_PSEAE
Q4KEB8, RTCA_PSEF5	Q9V0Z6, RTCA_PYRAB	Q8ZST4, RTCA_PYRAE
A3MXQ7, RTCA_PYRCJ	O59198, RTCA_PYRHO	A1RSJ7, RTCA_PYRIL
B1YDJ1, RTCA_PYRNV	Q8Y2V6, RTCA_RALN1	C3NMT8, RTCA_SACI1
C3MKC1, RTCA_SACI2	C3N179, RTCA_SACI3	C3MU91, RTCA_SACI4
C4KKE6, RTCA_SACI6	C3N969, RTCA_SACI7	Q97W04, RTCA_SACS2
B5F8N4, RTCA_SALA4	B5R378, RTCA_SALEP	B5R7J6, RTCA_SALG2
B4TKU5, RTCA_SALHS	C0Q0J3, RTCA_SALPC	Q8ZLI0, RTCA_SALTY
B2U3M8, RTCA_SHIB3	Q31VL3, RTCA_SHIBS	Q32AN4, RTCA_SHIDS
Q3YWK6, RTCA_SHISS	A3DN24, RTCA_STAMF	Q4JB78, RTCA_SULAC
Q974U1, RTCA_SULTO	C5A2A3, RTCA_THEGJ	Q5JIQ0, RTCA_THEKO
B6YSJ5, RTCA_THEON	C6A4N4, RTCA_THESM	Q3M6L1, RTCA_TRIV2

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.5.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.5.-.-
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