- The enzyme is a 3'-5' nucleic acid ligase with the ability to join
RNA with 3'-phosphate or 2',3'-cyclic-phosphate ends to RNA with
- It can also join DNA with 3'-phosphate ends to DNA with 5'-hydroxy
ends, provided the DNA termini are unpaired.
- The enzyme is found in members of all three kingdoms of life, and is
essential in metazoa for the splicing of intron-containing tRNAs.
- The reaction follows a three-step mechanism with initial activation
of the enzyme by GTP hydrolysis, forming a phosphoramide bond between
the guanylate and a histidine residue.
- The guanylate group is transferred to the 3'-phosphate terminus of
the substrate, forming the capped structure (DNA/RNA)-
- When a suitable 5'-OH end is available, the enzyme catalyzes an
attack of the 5'-OH on the capped end to form a 3'-5' phosphodiester
splice junction, releasing the guanylate.
- When acting on an RNA 2',3'-cyclic-phosphate, the enzyme catalyzes an
additional reaction, hydrolyzing the cyclic phosphate to a
- The metazoan enzyme requires activating cofactors in order to achieve
multiple turnover catalysis.
|B0WCT9, RTCB1_CULQU; ||B0EAV2, RTCB1_ENTDS; ||C4M244, RTCB1_ENTHI; |
|B0XKF3, RTCB2_CULQU; ||B0EIW5, RTCB2_ENTDS; ||C4M6T2, RTCB2_ENTHI; |
|Q17FP1, RTCB_AEDAE; ||Q9YB37, RTCB_AERPE; ||Q7Q412, RTCB_ANOGA; |
|O29399, RTCB_ARCFU; ||Q5E9T9, RTCB_BOVIN; ||C3YN79, RTCB_BRAFL; |
|A8QC60, RTCB_BRUMA; ||P90838, RTCB_CAEEL; ||A8JC00, RTCB_CHLRE; |
|Q6NZS4, RTCB_DANRE; ||Q54Y09, RTCB_DICDI; ||Q9VIW7, RTCB_DROME; |
|P46850, RTCB_ECOLI; ||Q9Y3I0, RTCB_HUMAN; ||Q4R6X4, RTCB_MACFA; |
|Q58095, RTCB_METJA; ||Q8TUS2, RTCB_METKA; ||Q6LXF9, RTCB_METMP; |
|O27634, RTCB_METTH; ||C1E9Y5, RTCB_MICCC; ||C1MI97, RTCB_MICPC; |
|A9UXG6, RTCB_MONBE; ||Q99LF4, RTCB_MOUSE; ||P59975, RTCB_MYCBO; |
|P9WGW4, RTCB_MYCTO; ||P9WGW5, RTCB_MYCTU; ||Q74MJ0, RTCB_NANEQ; |
|A7RKF6, RTCB_NEMVE; ||A4S3S3, RTCB_OSTLU; ||Q00ZY2, RTCB_OSTTA; |
|A9CB42, RTCB_PAPAN; ||Q19PY3, RTCB_PIG; ||Q4YUZ9, RTCB_PLABA; |
|Q8IIU6, RTCB_PLAF7; ||B3L4K9, RTCB_PLAKH; ||Q7RI54, RTCB_PLAYO; |
|Q9V168, RTCB_PYRAB; ||Q8ZY09, RTCB_PYRAE; ||Q8U0H4, RTCB_PYRFU; |
|O59245, RTCB_PYRHO; ||Q6AYT3, RTCB_RAT; ||B8LBM8, RTCB_THAPS; |
|Q4U923, RTCB_THEAN; ||Q5JCZ1, RTCB_THEKO; ||Q4N1R8, RTCB_THEPA; |
|B3RID0, RTCB_TRIAD; ||Q561P3, RTCB_XENTR; |