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ENZYME

ENZYME entry: EC 6.6.1.2

PURL: https://purl.expasy.org/enzyme/EC/6.6.1.2
Accepted Name
cobaltochelatase
Alternative Name(s)
hydrogenobyrinic acid a,c-diamide cobaltochelatase
Reaction catalysed
hydrogenobyrinate a,c-diamide + Co(2+) + ATP + H2O = cob(II)yrinate a,c diamide + ADP + phosphate + 5 H(+)
Comment(s)
  • This enzyme, which forms part of the aerobic (late cobalt insertion) cobalamin biosynthesis pathway, is a type I chelatase, being heterotrimeric and ATP-dependent.
  • It comprises two components, one of which corresponds to CobN and the other is composed of two polypeptides, specified by cobS and cobT in Pseudomonas denitrificans, and named CobST.
  • Hydrogenobyrinate is a very poor substrate.
  • ATP can be replaced by dATP or CTP but the reaction proceeds more slowly.
  • CobN exhibits a high affinity for hydrogenobyrinate a,c-diamide.
  • The oligomeric protein CobST possesses at least one sulfhydryl group that is essential for ATP-binding.
  • See EC 4.99.1.3 for the cobaltochelatase that participates in the anaerobic cobalamin biosynthesis pathway.
Cross-references
BRENDA6.6.1.2
EC2PDB6.6.1.2
ExplorEnz6.6.1.2
KEGG Ligand Database for Enzyme Nomenclature6.6.1.2
IUBMB Enzyme Nomenclature6.6.1.2
MEDLINEFind literature relating to 6.6.1.2
MetaCyc6.6.1.2
Rhea expert-curated reactions6.6.1.2
UniProtKB/Swiss-Prot
Q9HZQ3, COBN_PSEAEP29929, COBN_SINSXP29933, COBS_SINSX
P29934, COBT_SINSX

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