ENZYME entry: EC 1.14.19.9

Accepted Name

tryptophan 7-halogenase

Reaction catalysed

chloride + FADH2 + L-tryptophan + O2 <=> 7-chloro-L-tryptophan + FAD + 2 H2O

Comment(s)

A flavin-dependent halogenase.
The enzyme from the bacterium Lechevalieria aerocolonigenes catalyzes the initial step in the biosynthesis of rebeccamycin.
It utilizes molecular oxygen to oxidize the FADH2 cofactor, giving C4a-hydroperoxyflavin, which then reacts with chloride to produce a hypochlorite ion.
The latter reacts with an active site lysine to generate a chloramine, which chlorinates the substrate.
Also acts on bromide ion. cf. EC 1.14.19.58 and EC 1.14.19.59.
Formerly EC 1.14.14.7.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

IntEnz

MEDLINE

MetaCyc

Rhea expert-curated reactions

UniProtKB/Swiss-Prot

P95480, PRNA_PSEFL

Q8KHZ8, REBH_LENAE

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.

All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.19.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-