ENZYME entry: EC 1.8.1.4

Accepted Name

dihydrolipoyl dehydrogenase

Alternative Name(s)

dehydrolipoate dehydrogenase

diaphorase

dihydrolipoamide dehydrogenase

dihydrolipoic dehydrogenase

dihydrothioctic dehydrogenase

E3 component of alpha-ketoacid dehydrogenase complexes

glycine-cleavage system L-protein

LDP-Glc

LDP-Val

lipoamide dehydrogenase (NADH)

lipoamide oxidoreductase (NADH)

lipoamide reductase

lipoamide reductase (NADH)

lipoate dehydrogenase

lipoic acid dehydrogenase

lipoyl dehydrogenase

L-protein

Reaction catalysed

N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = H(+) + N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH

Comment(s)

A component of the multienzyme 2-oxo-acid dehydrogenase complexes.
In the pyruvate dehydrogenase complex, it binds to the core of EC 2.3.1.12 and catalyzes oxidation of its dihydrolipoyl groups.
It plays a similar role in the oxoglutarate and 3-methyl-2- oxobutanoate dehydrogenase complexes.
Another substrate is the dihydrolipoyl group in the H-protein of the glycine-cleavage system, in which it acts, together with EC 1.4.4.2 and EC 2.1.2.10 to break down glycine.
It can also use free dihydrolipoate, dihydrolipoamide or dihydrolipoyllysine as substrate.
Was first shown to catalyze the oxidation of NADH by methylene blue; this activity was called diaphorase.
The glycine cleavage system is composed of four components that only loosely associate: the P protein (EC 1.4.4.2), the T protein (EC 2.1.2.10), the L protein (EC 1.8.1.4) and the lipoyl-bearing H protein.
Formerly EC 1.6.4.3.

Cross-references

BRENDA

1.8.1.4

EC2PDB

1.8.1.4

ExplorEnz

1.8.1.4

PRIAM enzyme-specific profiles

1.8.1.4

KEGG Ligand Database for Enzyme Nomenclature

1.8.1.4

IUBMB Enzyme Nomenclature

1.8.1.4

IntEnz

1.8.1.4

MEDLINE

Find literature relating to 1.8.1.4

MetaCyc

1.8.1.4

Rhea expert-curated reactions

1.8.1.4

UniProtKB/Swiss-Prot

Q9M5K3, DLDH1_ARATH	P21880, DLDH1_BACSU	P11959, DLDH1_GEOSE
Q9I1L9, DLDH1_PSEAE	P09063, DLDH1_PSEPU	Q9M5K2, DLDH2_ARATH
P54533, DLDH2_BACSU	Q5UYG6, DLDH2_HALMA	Q9I3D1, DLDH2_PSEAE
P31052, DLDH2_PSEPU	O34324, DLDH3_BACSU	Q5UWH2, DLDH3_HALMA
Q9HUY1, DLDH3_PSEAE	P31046, DLDH3_PSEPU	P35484, DLDH_ACHLA
P18925, DLDH_AZOVI	F1N206, DLDH_BOVIN	P57303, DLDH_BUCAI
Q8K9T7, DLDH_BUCAP	Q89AQ8, DLDH_BUCBP	O17953, DLDH_CAEEL
P49819, DLDH_CANLF	G0SB20, DLDH_CHATD	Q9PJI3, DLDH_CHLMU
O50311, DLDH_CHLP8	Q9Z773, DLDH_CHLPN	Q8KCW2, DLDH_CHLTE
O84561, DLDH_CHLTR	Q8NTE1, DLDH_CORGL	Q8CIZ7, DLDH_CRIGR
P52992, DLDH_CUPNH	Q54EW8, DLDH_DICDI	P0A9P2, DLDH_ECO57
P0A9P1, DLDH_ECOL6	P0A9P0, DLDH_ECOLI	P43784, DLDH_HAEIN
Q9HN74, DLDH_HALSA	Q04829, DLDH_HALVD	P09622, DLDH_HUMAN
P80647, DLDH_HYMDI	Q60HG3, DLDH_MACFA	O18480, DLDH_MANSE
Q811C4, DLDH_MESAU	O08749, DLDH_MOUSE	P66005, DLDH_MYCBO
P47513, DLDH_MYCGE	Q50068, DLDH_MYCLE	P75393, DLDH_MYCPN
P9WHH8, DLDH_MYCTO	P9WHH9, DLDH_MYCTU	P31023, DLDH_PEA
P09623, DLDH_PIG	Q5R4B1, DLDH_PONAB	P84545, DLDH_POPEU
P14218, DLDH_PSEFL	Q6P6R2, DLDH_RAT	O05940, DLDH_RHIEC
P95596, DLDH_RHOCA	O00087, DLDH_SCHPO	P0A9P3, DLDH_SHIFL
P80503, DLDH_SOLTU	Q5HGY8, DLDH_STAAC	P0A0E6, DLDH_STAAM
P99084, DLDH_STAAN	Q6GHY9, DLDH_STAAR	Q6GAB8, DLDH_STAAS
P0A0E8, DLDH_STAAU	P0A0E7, DLDH_STAAW	P72740, DLDH_SYNY3
P85207, DLDH_THESS	Q04933, DLDH_TRYBB	P90597, DLDH_TRYCR
Q9KPF6, DLDH_VIBCH	O50286, DLDH_VIBPA	P09624, DLDH_YEAST
P50970, DLDH_ZYMMO	A8MS68, PLPD1_ARATH	F4JLP5, PLPD2_ARATH

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