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ENZYME entry: EC

Accepted Name
E2 NEDD8-conjugating enzyme.
Alternative Name(s)
NEDD8-carrier-protein E2.
NEDD8-conjugating enzyme E2.
Reaction catalysed
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine <=> [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine
  • Some RING-type E3 ubiquitin transferases (EC are not able to bind a substrate protein directly.
  • Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING- Ligase) complexes.
  • The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation.
  • Like ubiquitin, the NEDD8 protein ends with two glycine residues.
  • EC activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues.
  • The activated NEDD8 is subsequently transferred to a cysteine residue of an E2 NEDD8-conjugating enzyme, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC
PRIAM enzyme-specific profiles2.3.2.34
KEGG Ligand Database for Enzyme Nomenclature2.3.2.34
IUBMB Enzyme Nomenclature2.3.2.34
MEDLINEFind literature relating to
Rhea expert-curated reactions2.3.2.34
Q54TI6, UBC12_DICDI;  P61081, UBC12_HUMAN;  Q6CSW8, UBC12_KLULA;  
P61082, UBC12_MOUSE;  O74549, UBC12_SCHPO;  Q6DCZ9, UBC12_XENLA;  
Q6P8D9, UBC12_XENTR;  Q6C9W0, UBC12_YARLI;  P52491, UBC12_YEAST;  

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