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ENZYME entry: EC 6.2.1.64

Accepted Name
E1 NEDD8-activating enzyme.
Alternative Name(s)
NEDD-activating enzyme E1.
Reaction catalysed
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine <=> AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
Comment(s)
  • Some RING-type E3 ubiquitin transferase (EC 2.3.2.27) are not able to bind a substrate protein directly.
  • Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING- Ligase) complexes.
  • The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation.
  • Like ubiquitin, the NEDD8 protein ends with two glycine residues.
  • The E1 NEDD8-activating enzyme activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues.
  • The activated NEDD8 is subsequently transferred to a cysteine residue of EC 2.3.2.34, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC 2.3.2.32).
Cross-references
BRENDA6.2.1.64
EC2PDB6.2.1.64
ExplorEnz6.2.1.64
PRIAM enzyme-specific profiles6.2.1.64
KEGG Ligand Database for Enzyme Nomenclature6.2.1.64
IUBMB Enzyme Nomenclature6.2.1.64
IntEnz6.2.1.64
MEDLINEFind literature relating to 6.2.1.64
MetaCyc6.2.1.64
Rhea expert-curated reactions6.2.1.64
UniProtKB/Swiss-Prot
O65041, UBA3_ARATH;  Q19360, UBA3_CAEEL;  Q7ZVX6, UBA3_DANRE;  
Q54QG9, UBA3_DICDI;  Q9V6U8, UBA3_DROME;  Q8TBC4, UBA3_HUMAN;  
Q8C878, UBA3_MOUSE;  Q5R4A0, UBA3_PONAB;  Q99MI7, UBA3_RAT;  
Q09765, UBA3_SCHPO;  Q99344, UBA3_YEAST;  

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