ENZYME entry: EC 3.4.22.61

Accepted Name

caspase-8

Alternative Name(s)

apoptotic cysteine protease

apoptotic protease Mch-5

CAP4

CASP-8

FADD-homologous ICE/CED-3-like protease

FADD-like ICE

FLICE

ICE-like apoptotic protease 5

MACH

mammalian Ced-3 homolog 5

Mch5

MORT1-associated CED-3 homolog

Reaction catalysed

Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala)

Comment(s)

Caspase-8 is an initiator caspase, as are caspase-2 (EC 3.4.22.55), caspase-9 (EC 3.4.22.62) and caspase-10 (EC 3.4.22.63).
Apical activator of the extrinsic (death receptor) apoptosis pathway, triggered by death receptor ligation.
Contains two tandem death effector domains (DEDs) in its N-terminal prodomain, which play a role in procaspase activation.
Linked to cell surface death receptors such as Fas.
When Fas is aggregated by the Fas ligand, procaspase-8 is recruited to the death receptor where it is activated.
Has a preference for Glu at P3 and prefers small residues, such as Gly, Ser and Ala at the P1' position.
Has very broad P4 specificity, tolerating substrates with Asp, Val or Leu in this position.
Endogenous substrates for caspase-8 include procaspase-3, the pro- apoptotic Bcl-2 family member Bid, RIP, PAK2 and the caspase-8 activity modulator FLIPL.
Belongs to peptidase family C14.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

IntEnz

MEDLINE

MetaCyc

Rhea expert-curated reactions

UniProtKB/Swiss-Prot

Q8IRY7, CASP8_DROME	Q29IM7, CASP8_DROPS	Q14790, CASP8_HUMAN
O89110, CASP8_MOUSE	Q9JHX4, CASP8_RAT

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.

All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.4.22.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.4.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.-.-.-