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ENZYME

ENZYME entry: EC 3.5.1.87

PURL: https://purl.expasy.org/enzyme/EC/3.5.1.87
Accepted Name
N-carbamoyl-L-amino-acid hydrolase
Alternative Name(s)
L-carbamoylase
L-N-carbamoylase
N-carbamoylase
N-carbamoyl L-amino acid amidohydrolase
N-carbamoyl-L-amino acid amidohydrolase
Reaction catalysed
an N-carbamoyl-L-alpha-amino acid + H2O + 2 H(+) = an L-alpha-amino acid + NH4(+) + CO2
Comment(s)
  • This enzyme, along with EC 3.5.1.77, EC 5.1.99.5 and EC 3.5.2.2, forms part of the reaction cascade known as the 'hydantoinase process', which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids.
  • The enzyme from Alcaligenes xylosoxidans has broad specificity for carbamoyl-L-amino acids, although it is inactive on the carbamoyl derivatives of glutamate, aspartate, arginine, tyrosine or tryptophan.
  • The enzyme from Sinorhizobium meliloti requires a divalent cation for activity and can hydrolyze N-carbamoyl-L-tryptophan as well as N-carbamoyl L-amino acids with aliphatic substituents.
  • The enzyme is inactive on derivatives of D-amino acids.
  • In addition to N-carbamoyl L-amino acids, the enzyme can also hydrolyze formyl and acetyl derivatives to varying degrees.
Cross-references
BRENDA3.5.1.87
EC2PDB3.5.1.87
ExplorEnz3.5.1.87
KEGG Ligand Database for Enzyme Nomenclature3.5.1.87
IUBMB Enzyme Nomenclature3.5.1.87
MEDLINEFind literature relating to 3.5.1.87
MetaCyc3.5.1.87
Rhea expert-curated reactions3.5.1.87
UniProtKB/Swiss-Prot
P37113, AMAB1_GEOSEQ53389, AMAB2_GEOSEQ9F464, HYUC_PAEAU
Q01264, HYUC_PSESNQ6DTN4, HYUC_RHIML

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.5.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.-.-.-