ENZYME entry: EC 5.1.99.5
Accepted Name |
Hydantoin racemase.
|
Alternative Name(s) |
5'-monosubstituted-hydantoin racemase. |
Reaction catalysed |
D-5-monosubstituted hydantoin <=> L-5-monosubstituted hydantoin |
Comment(s) |
- This enzyme, along with EC 3.5.1.77, EC 3.5.1.87 and EC 3.5.2.2,
forms part of the reaction cascade known as the 'hydantoinase
process', which allows the total conversion of D,L-5-monosubstituted
hydantoins into optically pure D- or L-amino acids.
- The enzyme from Pseudomonas sp. (HyuE) has a preference for
hydantoins with aliphatic substituents, e.g. D- and L-5-
(2-(methylsulfanyl)ethyl)hydantoin, whereas that from Arthrobacter
aurescens shows highest activity with arylalkyl substituents,
especially 5-benzylhydantoin, at the 5-position.
- In the enzyme from Sinorhizobium meliloti, Cys-76 is responsible for
recognition and proton retrieval of D-isomers, while Cys-181 is
responsible for L-isomer recognition and racemization.
|
Cross-references |
BRENDA | 5.1.99.5 |
EC2PDB | 5.1.99.5 |
ExplorEnz | 5.1.99.5 |
PRIAM enzyme-specific profiles | 5.1.99.5 |
KEGG Ligand Database for Enzyme Nomenclature | 5.1.99.5 |
IUBMB Enzyme Nomenclature | 5.1.99.5 |
IntEnz | 5.1.99.5 |
MEDLINE | Find literature relating to 5.1.99.5 |
MetaCyc | 5.1.99.5 |
Rhea expert-curated reactions | 5.1.99.5 |
UniProtKB/Swiss-Prot |
|
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