ENZYME entry: EC 5.4.4.5

Accepted Name

9,12-octadecadienoate 8-hydroperoxide 8R-isomerase

Alternative Name(s)

(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate mutase ((5S,8R,9Z,12Z)- 5,8-dihydroxy-9,12-octadecadienoate-forming)

Reaction catalysed

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate = (5S,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate

Comment(s)

The bifunctional enzyme from Aspergillus nidulans uses different heme domains to catalyze two separate reactions.
Linoleic acid is oxidized within the N-terminal heme peroxidase domain to (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate (cf. EC 1.13.11.60), which is subsequently isomerized to (5S,8R,9Z,12Z)- 5,8-dihydroxyoctadeca-9,12-dienoate within the C-terminal P450 heme thiolate domain.

Cross-references

BRENDA

EC2PDB

ExplorEnz

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

MEDLINE

MetaCyc

Rhea expert-curated reactions

UniProtKB/Swiss-Prot

B0Y6R2, PPOA_ASPFC	Q4WPX2, PPOA_ASPFU	Q6RET3, PPOA_EMEND
G5EB19, PPOA_EMENI

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.

All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 5.4.4.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 5.4.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 5.-.-.-