Home  |  Contact

ENZYME entry: EC

Accepted Name
Glutamate--tRNA(Gln) ligase.
Alternative Name(s)
Nondiscriminating glutamyl-tRNA synthetase.
Reaction catalysed
ATP + L-glutamate + tRNA(Glx) <=> AMP + diphosphate + L-glutamyl-tRNA(Glx)
  • When this enzyme acts on tRNA(Glu), it catalyzes the same reaction as EC
  • It has, however, diminished discrimination, so that it can also form glutamate-tRNA(Gln).
  • This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon.
  • This accounts for the ability of this enzyme in, for example, Bacillus subtilis, to recognize both tRNA(1)(Gln) (UUG anticodon) and tRNA(Glu) (UUC anticodon) but not tRNA(2)(Gln) (CUG anticodon).
  • The ability of this enzyme to recognize both tRNA(Glu) and one of the tRNA(Gln) isoacceptors derives from their sharing a major identity element, a hypermodified derivative of U34 (5-methylaminomethyl-2- thiouridine).
  • The glutamyl-tRNA(Gln) is not used in protein synthesis until it is converted by EC into glutaminyl-tRNA(Gln).
PRIAM enzyme-specific profiles6.1.1.24
KEGG Ligand Database for Enzyme Nomenclature6.1.1.24
IUBMB Enzyme Nomenclature6.1.1.24
MEDLINEFind literature relating to

View entry in original ENZYME format
View entry in raw text format (no links)
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.1.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.1.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-