ENZYME entry: EC 6.1.1.24
| Accepted Name |
|---|
| Glutamate--tRNA(Gln) ligase.
|
| Alternative Name(s) |
|---|
| Nondiscriminating glutamyl-tRNA synthetase. |
| Reaction catalysed |
|---|
| ATP + L-glutamate + tRNA(Glx) <=> AMP + diphosphate + L-glutamyl-tRNA(Glx) |
| Comment(s) |
|---|
- When this enzyme acts on tRNA(Glu), it catalyzes the same reaction as
EC 6.1.1.17.
- It has, however, diminished discrimination, so that it can also form
glutamate-tRNA(Gln).
- This relaxation of specificity has been found to result from the
absence of a loop in the tRNA that specifically recognizes the third
position of the anticodon.
- This accounts for the ability of this enzyme in, for example,
Bacillus subtilis, to recognize both tRNA(1)(Gln) (UUG anticodon) and
tRNA(Glu) (UUC anticodon) but not tRNA(2)(Gln) (CUG anticodon).
- The ability of this enzyme to recognize both tRNA(Glu) and one of the
tRNA(Gln) isoacceptors derives from their sharing a major identity
element, a hypermodified derivative of U34 (5-methylaminomethyl-2-
thiouridine).
- The glutamyl-tRNA(Gln) is not used in protein synthesis until it is
converted by EC 6.3.5.7 into glutaminyl-tRNA(Gln).
|
| Cross-references |
|---|
| BRENDA | 6.1.1.24 |
| EC2PDB | 6.1.1.24 |
| ExplorEnz | 6.1.1.24 |
| PRIAM enzyme-specific profiles | 6.1.1.24 |
| KEGG Ligand Database for Enzyme Nomenclature | 6.1.1.24 |
| IUBMB Enzyme Nomenclature | 6.1.1.24 |
| IntEnz | 6.1.1.24 |
| MEDLINE | Find literature relating to 6.1.1.24 |
| MetaCyc | 6.1.1.24 |
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