ENZYME

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A new class EC 7, Translocases, has been added to the EC list. It will be part of ENZYME from release 2018_10. Read more about EC 7 here.

ENZYME entry: EC 6.1.1.24

Accepted Name
Glutamate--tRNA(Gln) ligase.
Alternative Name(s)
Nondiscriminating glutamyl-tRNA synthetase.
Reaction catalysed
ATP + L-glutamate + tRNA(Glx) <=> AMP + diphosphate + L-glutamyl-tRNA(Glx)
Comment(s)
When this enzyme acts on tRNA(Glu), it catalyzes the same reaction as EC 6.1.1.17. It has, however, diminished discrimination, so that it can also form glutamate-tRNA(Gln). This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon. This accounts for the ability of this enzyme in, for example, Bacillus subtilis, to recognize both tRNA(1)(Gln) (UUG anticodon) and tRNA(Glu) (UUC anticodon) but not tRNA(2)(Gln) (CUG anticodon). The ability of this enzyme to recognize both tRNA(Glu) and one of the tRNA(Gln) isoacceptors derives from their sharing a major identity element, a hypermodified derivative of U34 (5-methylaminomethyl-2- thiouridine). The glutamyl-tRNA(Gln) is not used in protein synthesis until it is converted by EC 6.3.5.7 into glutaminyl-tRNA(Gln).
Cross-references
BRENDA	6.1.1.24
EC2PDB	6.1.1.24
ExplorEnz	6.1.1.24
PRIAM enzyme-specific profiles	6.1.1.24
KEGG Ligand Database for Enzyme Nomenclature	6.1.1.24
IUBMB Enzyme Nomenclature	6.1.1.24
IntEnz	6.1.1.24
MEDLINE	Find literature relating to 6.1.1.24
MetaCyc	6.1.1.24