ENZYME

ENZYME entry: EC 6.3.2.14

Accepted Name

Enterobactin synthase.

Alternative Name(s)

2,3-dihydroxybenzoate--serine ligase.

N-(2,3-dihydroxybenzoyl)-serine synthetase.

Reaction catalysed

6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine <=> enterobactin + 6 AMP + 6 diphosphate

Comment(s)

This enzyme complex catalyzes the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin.
In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyzes the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyzes the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3- dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule).

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature