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ENZYME

ENZYME entry: EC 6.3.2.17

PURL: https://purl.expasy.org/enzyme/EC/6.3.2.17
Accepted Name
tetrahydrofolate synthase
Alternative Name(s)
folate polyglutamate synthetase
folylpoly(gamma-glutamate) synthase
folylpoly-gamma-glutamate synthase
folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase
folylpolyglutamate synthase
folylpolyglutamate synthetase
folylpolyglutamyl synthetase
formyltetrahydropteroyldiglutamate synthetase
FPGS
N(10)-formyltetrahydropteroyldiglutamate synthetase
tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming)
tetrahydrofolylpolyglutamate synthase
Reaction catalysed
(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)
Comment(s)
  • In some bacteria, a single protein catalyzes both this activity and that of EC 6.3.2.12, the combined activity of which leads to the formation of the cofactor polyglutamated tetrahydropteroate (H4PteGlun), i.e. various tetrahydrofolates (H4folate).
  • In contrast, the activities are located on separate proteins in most eukaryotes studied to date.
  • In Arabidopsis thaliana, this enzyme is present as distinct isoforms in the mitochondria, the cytosol and the chloroplast.
  • Each isoform is encoded by a separate gene, a situation that is unique among eukaryotes.
  • As the affinity of folate-dependent enzymes increases markedly with the number of glutamic residues, the tetrahydropteroyl polyglutamates are the preferred cofactors of C1 metabolism.
  • The enzymes from different sources (particularly eukaryotes versus prokaryotes) have different substrate specificities with regard to one-carbon substituents and the number of glutamate residues present on the tetrahydrofolates.
Cross-references
BRENDA6.3.2.17
EC2PDB6.3.2.17
ExplorEnz6.3.2.17
KEGG Ligand Database for Enzyme Nomenclature6.3.2.17
IUBMB Enzyme Nomenclature6.3.2.17
MEDLINEFind literature relating to 6.3.2.17
MetaCyc6.3.2.17
Rhea expert-curated reactions6.3.2.17
UniProtKB/Swiss-Prot
Q9HS44, FOLCP_HALSAD4GW05, FOLCP_HALVDQ05865, FOLC_BACSU
A6H751, FOLC_BOVINP57265, FOLC_BUCAIQ8K9X3, FOLC_BUCAP
Q89AT2, FOLC_BUCBPQ09509, FOLC_CAEELQ924L9, FOLC_CRIGR
Q54CY5, FOLC_DICDIP08192, FOLC_ECOLIP43775, FOLC_HAEIN
Q05932, FOLC_HUMANP48760, FOLC_MOUSEI6Y0R5, FOLC_MYCTU
P36001, FOLC_YEASTQ9Y893, FOLE_CANAXO13492, FOLE_NEUCR
O74742, FOLE_SCHPOB3LJR0, FOLE_YEAS1B5VSC3, FOLE_YEAS6
A6ZP80, FOLE_YEAS7C8ZGZ3, FOLE_YEAS8E7KIA3, FOLE_YEASA
E7Q9C7, FOLE_YEASBE7KUJ4, FOLE_YEASLE7NMM0, FOLE_YEASO
Q08645, FOLE_YEASTE7QKX4, FOLE_YEASZF4K2A1, FPGS1_ARATH
F4J2K2, FPGS2_ARATHQ8W035, FPGS3_ARATHP15925, FPGS_LACCA

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