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ENZYME entry: EC 6.3.2.17

Accepted Name
tetrahydrofolate synthase.
Alternative Name(s)
folate polyglutamate synthetase.
folylpoly(gamma-glutamate) synthase.
folylpoly-gamma-glutamate synthase.
folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase.
folylpolyglutamate synthase.
folylpolyglutamate synthetase.
folylpolyglutamyl synthetase.
formyltetrahydropteroyldiglutamate synthetase.
FPGS.
N(10)-formyltetrahydropteroyldiglutamate synthetase.
tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming).
tetrahydrofolylpolyglutamate synthase.
Reaction catalysed
(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate <=> (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate
Comment(s)
  • In some bacteria, a single protein catalyzes both this activity and that of EC 6.3.2.12, the combined activity of which leads to the formation of the coenzyme polyglutamated tetrahydropteroate (H4PteGlun), i.e. various tetrahydrofolates (H4folate).
  • In contrast, the activities are located on separate proteins in most eukaryotes studied to date.
  • In Arabidopsis thaliana, this enzyme is present as distinct isoforms in the mitochondria, the cytosol and the chloroplast.
  • Each isoform is encoded by a separate gene, a situation that is unique among eukaryotes.
  • As the affinity of folate-dependent enzymes increases markedly with the number of glutamic residues, the tetrahydropteroyl polyglutamates are the preferred coenzymes of C1 metabolism.
  • The enzymes from different sources (particularly eukaryotes versus prokaryotes) have different substrate specificities with regard to one-carbon substituents and the number of glutamate residues present on the tetrahydrofolates.
Cross-references
BRENDA6.3.2.17
EC2PDB6.3.2.17
ExplorEnz6.3.2.17
PRIAM enzyme-specific profiles6.3.2.17
KEGG Ligand Database for Enzyme Nomenclature6.3.2.17
IUBMB Enzyme Nomenclature6.3.2.17
IntEnz6.3.2.17
MEDLINEFind literature relating to 6.3.2.17
MetaCyc6.3.2.17
Rhea expert-curated reactions6.3.2.17
UniProtKB/Swiss-Prot
Q9HS44, FOLCP_HALSA;  D4GW05, FOLCP_HALVD;  Q05865, FOLC_BACSU;  
A6H751, FOLC_BOVIN;  P57265, FOLC_BUCAI;  Q8K9X3, FOLC_BUCAP;  
Q89AT2, FOLC_BUCBP;  Q09509, FOLC_CAEEL;  Q924L9, FOLC_CRIGR;  
Q54CY5, FOLC_DICDI;  P08192, FOLC_ECOLI;  P43775, FOLC_HAEIN;  
Q05932, FOLC_HUMAN;  P48760, FOLC_MOUSE;  I6Y0R5, FOLC_MYCTU;  
P36001, FOLC_YEAST;  Q9Y893, FOLE_CANAX;  O13492, FOLE_NEUCR;  
O74742, FOLE_SCHPO;  B3LJR0, FOLE_YEAS1;  B5VSC3, FOLE_YEAS6;  
A6ZP80, FOLE_YEAS7;  C8ZGZ3, FOLE_YEAS8;  E7KIA3, FOLE_YEASA;  
E7Q9C7, FOLE_YEASB;  E7KUJ4, FOLE_YEASL;  E7NMM0, FOLE_YEASO;  
Q08645, FOLE_YEAST;  E7QKX4, FOLE_YEASZ;  F4K2A1, FPGS1_ARATH;  
F4J2K2, FPGS2_ARATH;  Q8W035, FPGS3_ARATH;  P15925, FPGS_LACCA;  

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