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ENZYME

ENZYME entry: EC 6.3.4.16

Accepted Name
carbamoyl-phosphate synthase (ammonia)
Alternative Name(s)
carbamoylphosphate synthase (ammonia)
carbamoylphosphate synthetase (ammonia)
carbamoyl-phosphate synthetase (ammonia)
carbamoyl-phosphate synthetase I
carbon-dioxide--ammonia ligase
CPS I
Reaction catalysed
2 ATP + hydrogencarbonate + NH4(+) <=> 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate
Comment(s)
  • The enzyme catalyzes the first committed step in the urea cycle.
  • The reaction proceeds via three separate chemical reactions: phosphorylation of hydrogencarbonate to carboxyphosphate; a nucleophilic attack of ammonia on carboxyphosphate yielding carbamate; and the phosphorylation of carbamate forming carbamoyl phosphate.
  • Two moles of ATP are utilized for the synthesis of one molecule of carbamoyl phosphate, making the reaction essentially irreversible.
  • The enzyme requires the allosteric activator N-acetyl-L-glutamate. cf. EC 6.3.5.5.
  • Formerly EC 2.7.2.5.
Cross-references
BRENDA6.3.4.16
EC2PDB6.3.4.16
ExplorEnz6.3.4.16
PRIAM enzyme-specific profiles6.3.4.16
KEGG Ligand Database for Enzyme Nomenclature6.3.4.16
IUBMB Enzyme Nomenclature6.3.4.16
IntEnz6.3.4.16
MEDLINEFind literature relating to 6.3.4.16
MetaCyc6.3.4.16
Rhea expert-curated reactions6.3.4.16
UniProtKB/Swiss-Prot
O67869, CARB1_AQUAEQ58773, CARB1_METJAQ8TWX0, CARB1_METKA
O67233, CARB2_AQUAEQ58776, CARB2_METJAQ8TUT7, CARB2_METKA
C1F1S6, CARB_ACIC5C4ZEK2, CARB_AGARVQ8UDE9, CARB_AGRFC
Q42601, CARB_ARATHO28994, CARB_ARCFUA0JX72, CARB_ARTS2
C3P656, CARB_BACAAC3L740, CARB_BACACA0RHQ8, CARB_BACAH
Q81WF2, CARB_BACANB7JJX3, CARB_BACC0Q732I3, CARB_BACC1
B7IUP6, CARB_BACC2C1EPQ0, CARB_BACC3B7H6M2, CARB_BACC4
B7HLM0, CARB_BACC7P46537, CARB_BACCLA7GRL1, CARB_BACCN
B9IVW3, CARB_BACCQQ819S3, CARB_BACCRQ636E0, CARB_BACCZ
Q6HES8, CARB_BACHKA9VTC6, CARB_BACMKP25994, CARB_BACSU
C5C687, CARB_BEUC1B8DTW3, CARB_BIFA0A1A0T4, CARB_BIFAA
B3DQ32, CARB_BIFLDQ8G815, CARB_BIFLOB7GPW2, CARB_BIFLS
Q8YIC2, CARB_BRUMEQ8FZJ3, CARB_BRUSUP57244, CARB_BUCAI
Q8K9Z7, CARB_BUCAPP59448, CARB_BUCBPQ8RBK0, CARB_CALS4
Q9PIL7, CARB_CAMJEQ9A4D6, CARB_CAUVCA5CRX5, CARB_CLAM3
B0REV9, CARB_CLASEQ97FT3, CARB_CLOABA6LPD9, CARB_CLOB8
B2UX86, CARB_CLOBAB2THH3, CARB_CLOBBC1FNY3, CARB_CLOBJ
A7GE89, CARB_CLOBLB1KT07, CARB_CLOBMQ0TM79, CARB_CLOP1
Q8XHB3, CARB_CLOPEQ0SPY4, CARB_CLOPSQ8FT42, CARB_COREF
P58939, CARB_CORGLQ6A914, CARB_CUTAKP46056, CARB_CUTCT
C1CXR4, CARB_DEIDVQ1IWM0, CARB_DEIGDQ9RWK0, CARB_DEIRA
B1I4M8, CARB_DESAPP63737, CARB_ECO57Q8FLB0, CARB_ECOL6
P00968, CARB_ECOLIQ834E2, CARB_ENTFAQ75D66, CARB_EREGS
B1YIR9, CARB_EXIS2C4L5W3, CARB_EXISAQ8RG86, CARB_FUSNN
C1A4I5, CARB_GEMATO50302, CARB_GEOSEQ7VP67, CARB_HAEDU
Q8RSS3, CARB_HALERQ9K9V9, CARB_HALH5B8D1H3, CARB_HALOH
Q9HP43, CARB_HALSAQ9ZKT2, CARB_HELPJO25577, CARB_HELPY
A2BJL3, CARB_HYPBUA6WCC6, CARB_KINRDB2GI87, CARB_KOCRD
Q1IPK2, CARB_KORVEQ5FJC0, CARB_LACACB3WEF5, CARB_LACCB
Q047M8, CARB_LACDBA8YVZ3, CARB_LACH4Q74J34, CARB_LACJO
Q9CFV2, CARB_LACLAO32771, CARB_LACLMQ02YG5, CARB_LACLS
Q038Z2, CARB_LACP3A9KI94, CARB_LACP7P77886, CARB_LACPL
Q38X24, CARB_LATSSQ72NF1, CARB_LEPICQ8F832, CARB_LEPIN
B1MZ74, CARB_LEUCKQ03WW7, CARB_LEUMMQ1WVA9, CARB_LIGS1
B2GD06, CARB_LIMF3A5VHN6, CARB_LIMRDB2G564, CARB_LIMRJ
Q92AH3, CARB_LISINC1KWD4, CARB_LISMCQ71YI1, CARB_LISMF
B8DDR7, CARB_LISMHQ8Y665, CARB_LISMOA0AJU0, CARB_LISW6
B1HQC1, CARB_LYSSCB9EB69, CARB_MACCJQ8TNY4, CARB_METAC
A7I6J1, CARB_METB6P13258, CARB_METBAQ469Z7, CARB_METBF
Q2FLD9, CARB_METHJA2SQ53, CARB_METLZP58944, CARB_METMA
A3CU73, CARB_METMJB8GEA3, CARB_METPEA5UKG5, CARB_METS3
O27077, CARB_METTHA0B8K9, CARB_METTPC5CCF1, CARB_MICLC
Q7U054, CARB_MYCBOA4TBY6, CARB_MYCGIQ9CCR2, CARB_MYCLE
A3PZ65, CARB_MYCSJA1UFK4, CARB_MYCSKQ1B9F9, CARB_MYCSS
P9WPK2, CARB_MYCTOP9WPK3, CARB_MYCTUA1T8H1, CARB_MYCVP
Q1D6Y8, CARB_MYXXDB2A170, CARB_NATTJQ59599, CARB_NEIGO
Q9JW02, CARB_NEIMAQ9JXW8, CARB_NEIMBQ7SH52, CARB_NEUCR
Q8YQL2, CARB_NOSS1Q04H29, CARB_OENOBB9EXM2, CARB_ORYSJ
A1R6Z3, CARB_PAEATQ9CKV0, CARB_PASMUQ03HB0, CARB_PEDPA
A5D508, CARB_PELTSP38100, CARB_PSEAEB8H8U5, CARB_PSECP
A8F453, CARB_PSELTQ88DU6, CARB_PSEPKQ87WP4, CARB_PSESM
Q8ZY48, CARB_PYRAEQ8U085, CARB_PYRFUQ8XZ83, CARB_RALN1
A9WSA8, CARB_RENSMQ98I87, CARB_RHILOQ92PZ4, CARB_RHIME
Q7UJ58, CARB_RHOBAQ1AVY9, CARB_RUBXDQ59969, CARB_SACS2
Q8Z9L7, CARB_SALTIP14846, CARB_SALTYO94313, CARB_SCHPO
P63738, CARB_SHIFLA7X1F4, CARB_STAA1A6U122, CARB_STAA2
Q2FHN5, CARB_STAA3Q2FZ72, CARB_STAA8A5IS88, CARB_STAA9
Q2YXG5, CARB_STAABQ5HGM9, CARB_STAACA6QGA4, CARB_STAAE
P63739, CARB_STAAMP63740, CARB_STAANQ6GHN2, CARB_STAAR
Q6GA10, CARB_STAASA8Z3P0, CARB_STAATP58940, CARB_STAAW
B9DPN2, CARB_STACTQ5HPY8, CARB_STAEQQ8CPJ4, CARB_STAES
Q4L5Q5, CARB_STAHJQ49WY4, CARB_STAS1Q3K150, CARB_STRA1
Q8E5F5, CARB_STRA3Q8DZQ7, CARB_STRA5Q827Q7, CARB_STRAW
Q9KXR6, CARB_STRCOC0M756, CARB_STRE4A8AX83, CARB_STRGC
B1W463, CARB_STRGGQ8DUP3, CARB_STRMUQ9A0C6, CARB_STRP1
Q04K48, CARB_STRP2P0DA14, CARB_STRP3B5E512, CARB_STRP4
Q5XCR6, CARB_STRP6C1C7Q3, CARB_STRP7P58941, CARB_STRP8
A2RF60, CARB_STRPGB1IC65, CARB_STRPIB8ZJT9, CARB_STRPJ
Q97QE4, CARB_STRPNP0DA15, CARB_STRPQB2IQ67, CARB_STRPS
B5XKW2, CARB_STRPZQ8CWR0, CARB_STRR6C0MEH1, CARB_STRS7
A3CNI5, CARB_STRSVQ5M0W9, CARB_STRT1Q5M5F6, CARB_STRT2
Q03LT8, CARB_STRTDB9DRV7, CARB_STRU0C1CEM9, CARB_STRZJ
C1CL09, CARB_STRZPC1CR31, CARB_STRZTQ4J8E8, CARB_SULAC
C3N664, CARB_SULIAC4KHM7, CARB_SULIKC3MQE3, CARB_SULIL
C3MW21, CARB_SULIMC3NH23, CARB_SULINC3NEM0, CARB_SULIY
Q970U7, CARB_SULTOQ67Q54, CARB_SYMTHQ55756, CARB_SYNY3
Q9HK17, CARB_THEACQ9WZ27, CARB_THEMAB9KB91, CARB_THENN
A5IJL8, CARB_THEP1B0KBW4, CARB_THEP3B0K4D7, CARB_THEPX
B9KXM5, CARB_THERPB1L8T8, CARB_THESQP96495, CARB_THET2
Q5SKN1, CARB_THET8Q97AJ3, CARB_THEVOQ9KPH9, CARB_VIBCH
Q87SF3, CARB_VIBPAQ8DEM2, CARB_VIBVUQ7MNU0, CARB_VIBVY
P58942, CARB_XANACP58943, CARB_XANCPQ9PEC1, CARB_XYLFA
Q87EB8, CARB_XYLFTP03965, CARB_YEASTQ8ZIL4, CARB_YERPE
O50236, CARB_ZYMMOP18185, CARY_BACSUQ9ZB63, CARY_GEOSE
Q9K8V7, CARY_HALH5Q9RLS9, CARY_LACPLQ91293, CPSM_AQUCT
P31327, CPSM_HUMANQ8C196, CPSM_MOUSEP07756, CPSM_RAT
A5UK38, CPS_METS3Q18990, PYR1_CAEELP20054, PYR1_DICDI
P05990, PYR1_DROMEO93937, PYR1_EMENIP27708, PYR1_HUMAN
P08955, PYR1_MESAUB2RQC6, PYR1_MOUSEQ7S8A6, PYR1_NEUCR
Q09794, PYR1_SCHPOQ91437, PYR1_SQUACP07259, PYR1_YEAST

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All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.3.4.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.3.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-