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ENZYME entry: EC 6.3.5.5

Accepted Name
carbamoyl-phosphate synthase (glutamine-hydrolyzing).
Alternative Name(s)
carbamoyl phosphate synthetase.
carbamoyl phosphate synthetase (glutamine).
carbamoyl-phosphate synthetase (glutamine-hydrolyzing).
carbamoylphosphate synthetase II.
CPS.
GD-CPSase.
glutamine-dependent carbamoyl-phosphate synthase.
glutamine-dependent carbamoyl phosphate synthetase.
Reaction catalysed
2 ATP + H2O + hydrogencarbonate + L-glutamine <=> 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate
Comment(s)
  • The product carbamoyl phosphate is an intermediate in the biosynthesis of arginine and the pyrimidine nucleotides.
  • The enzyme from Escherichia coli has three separate active sites, which are connected by a molecular tunnel that is almost 100 A in length.
  • The amidotransferase domain within the small subunit of the enzyme hydrolyzes glutamine to ammonia via a thioester intermediate.
  • The ammonia migrates through the interior of the protein, where it reacts with carboxyphosphate to produce the carbamate intermediate.
  • The carboxyphosphate intermediate is formed by the phosphorylation of hydrogencarbonate by ATP at a site contained within the N-terminal half of the large subunit.
  • The carbamate intermediate is transported through the interior of the protein to a second site within the C-terminal half of the large subunit, where it is phosphorylated by another ATP to yield the final product, carbamoyl phosphate.
  • Cf. EC 6.3.4.16.
  • Formerly EC 2.7.2.9.
Cross-references
BRENDA6.3.5.5
EC2PDB6.3.5.5
ExplorEnz6.3.5.5
PRIAM enzyme-specific profiles6.3.5.5
KEGG Ligand Database for Enzyme Nomenclature6.3.5.5
IUBMB Enzyme Nomenclature6.3.5.5
IntEnz6.3.5.5
MEDLINEFind literature relating to 6.3.5.5
MetaCyc6.3.5.5
Rhea expert-curated reactions6.3.5.5
UniProtKB/Swiss-Prot
Q6F8M7, CARA_ACIAD;  Q8UDF7, CARA_AGRFC;  B2URJ0, CARA_AKKM8;  
O66727, CARA_AQUAE;  Q9LVW7, CARA_ARATH;  O28995, CARA_ARCFU;  
Q752N9, CARA_ASHGO;  A1CA18, CARA_ASPCL;  Q4WU09, CARA_ASPFU;  
A2R9B9, CARA_ASPNC;  Q2U913, CARA_ASPOR;  Q81WF1, CARA_BACAN;  
Q732I2, CARA_BACC1;  P52557, CARA_BACCL;  Q819S2, CARA_BACCR;  
Q636D9, CARA_BACCZ;  Q6HES7, CARA_BACHK;  P25993, CARA_BACSU;  
A1UTE8, CARA_BARBK;  Q6G5M0, CARA_BARHE;  Q6FZ66, CARA_BARQU;  
A9IWF8, CARA_BART1;  B3DQ31, CARA_BIFLD;  Q8G816, CARA_BIFLO;  
B7GPW1, CARA_BIFLS;  Q89DR8, CARA_BRADU;  B2S6U9, CARA_BRUA1;  
Q2YM22, CARA_BRUA2;  A6WZJ5, CARA_BRUA4;  Q57C28, CARA_BRUAB;  
A9M6F1, CARA_BRUC2;  C0REC2, CARA_BRUMB;  Q8YIB8, CARA_BRUME;  
A5VRM1, CARA_BRUO2;  B0CHS0, CARA_BRUSI;  Q8FZJ8, CARA_BRUSU;  
P57245, CARA_BUCAI;  Q8K9Z6, CARA_BUCAP;  P59576, CARA_BUCBP;  
Q8RBK1, CARA_CALS4;  Q9PMG8, CARA_CAMJE;  Q5AML6, CARA_CANAL;  
Q6FQ30, CARA_CANGA;  Q9A4J7, CARA_CAUVC;  Q2H132, CARA_CHAGB;  
Q8KGA2, CARA_CHLTE;  Q97FT2, CARA_CLOAB;  Q8XHB2, CARA_CLOPE;  
Q1DUF5, CARA_COCIM;  Q6NH15, CARA_CORDI;  Q8FT41, CARA_COREF;  
P58893, CARA_CORGL;  O19886, CARA_CYACA;  Q6BJG4, CARA_DEBHA;  
Q1IWN0, CARA_DEIGD;  Q9RWI4, CARA_DEIRA;  B8IZS6, CARA_DESDA;  
Q726J4, CARA_DESVH;  Q8KZA0, CARA_DESVM;  A1VA26, CARA_DESVV;  
P0A6F2, CARA_ECO57;  Q8FLB1, CARA_ECOL6;  P0A6F1, CARA_ECOLI;  
Q5BB37, CARA_EMENI;  Q8RG87, CARA_FUSNN;  Q6B940, CARA_GRATL;  
Q7VP66, CARA_HAEDU;  Q8RSS4, CARA_HALER;  Q9K9V8, CARA_HALH5;  
B0R6F1, CARA_HALS3;  Q9HP42, CARA_HALSA;  Q17VI1, CARA_HELAH;  
Q7VHS5, CARA_HELHP;  Q9ZJY9, CARA_HELPJ;  O25835, CARA_HELPY;  
Q0BY73, CARA_HYPNA;  P87183, CARA_HYPVI;  Q6CIQ1, CARA_KLULA;  
Q9CF80, CARA_LACLA;  Q9L4N5, CARA_LACLM;  P77885, CARA_LACPL;  
Q1MPN3, CARA_LAWIP;  Q04R57, CARA_LEPBJ;  Q04ZG6, CARA_LEPBL;  
Q72PK5, CARA_LEPIC;  Q8F6R2, CARA_LEPIN;  Q92AH2, CARA_LISIN;  
Q71YI0, CARA_LISMF;  Q8Y664, CARA_LISMO;  Q0AMR3, CARA_MARMM;  
C6C0A9, CARA_MARSD;  Q8TNY3, CARA_METAC;  Q58425, CARA_METJA;  
Q8TY15, CARA_METKA;  A4G0Y3, CARA_METM5;  A9A972, CARA_METM6;  
A6VHH7, CARA_METM7;  Q8Q0U4, CARA_METMA;  Q6LWW5, CARA_METMP;  
A4YI77, CARA_METS5;  O27079, CARA_METTH;  A6UQN4, CARA_METVS;  
Q7U055, CARA_MYCBO;  Q9CCR3, CARA_MYCLE;  Q741H2, CARA_MYCPA;  
P9WPK4, CARA_MYCTO;  P9WPK5, CARA_MYCTU;  Q50983, CARA_NEIGO;  
Q9JVZ6, CARA_NEIMA;  Q9JXX4, CARA_NEIMB;  A1CZ92, CARA_NEOFI;  
Q1XDT6, CARA_NEOYE;  P22572, CARA_NEUCR;  Q8YXQ7, CARA_NOSS1;  
Q8CXH8, CARA_OCEIH;  Q6YV23, CARA_ORYSJ;  Q7U7F9, CARA_PARMW;  
Q9CKV3, CARA_PASMU;  Q6D0C8, CARA_PECAS;  Q0U5H7, CARA_PHANO;  
Q7N8W2, CARA_PHOLL;  Q6LUK6, CARA_PHOPR;  A4SXM1, CARA_POLAQ;  
B1XUM3, CARA_POLNS;  P51201, CARA_PORPU;  B4S5S1, CARA_PROA2;  
P38098, CARA_PSEAE;  Q88DU5, CARA_PSEPK;  Q87WP3, CARA_PSESM;  
P38099, CARA_PSEST;  Q8ZY49, CARA_PYRAE;  Q8U086, CARA_PYRFU;  
Q8XZ85, CARA_RALSO;  Q98IA7, CARA_RHILO;  Q92N95, CARA_RHIME;  
Q7UGJ6, CARA_RHOBA;  Q9JP87, CARA_RUBGI;  Q5LTN6, CARA_RUEPO;  
Q59968, CARA_SACS2;  Q8Z9L8, CARA_SALTI;  P14845, CARA_SALTY;  
O60060, CARA_SCHPO;  Q8EHS6, CARA_SHEON;  C4XRH8, CARA_SOLM1;  
Q5HGN0, CARA_STAAC;  P63729, CARA_STAAM;  P99147, CARA_STAAN;  
Q6GHN3, CARA_STAAR;  Q6GA11, CARA_STAAS;  P63730, CARA_STAAW;  
Q5HPY9, CARA_STAEQ;  Q8CPJ5, CARA_STAES;  Q4L5Q4, CARA_STAHJ;  
Q49WY3, CARA_STAS1;  P63731, CARA_STRA3;  P63732, CARA_STRA5;  
Q827Q8, CARA_STRAW;  Q9KXR5, CARA_STRCO;  Q8DUP4, CARA_STRMU;  
P63735, CARA_STRP1;  P0DA12, CARA_STRP3;  Q5XCR8, CARA_STRP6;  
P58894, CARA_STRP8;  P63733, CARA_STRPN;  P0DA13, CARA_STRPQ;  
P63734, CARA_STRR6;  Q4J8E9, CARA_SULAC;  Q970U8, CARA_SULTO;  
Q67Q55, CARA_SYMTH;  Q3A450, CARA_SYNC1;  Q31LB7, CARA_SYNE7;  
Q5N0L0, CARA_SYNP6;  A5GTM5, CARA_SYNR3;  P74587, CARA_SYNY3;  
C5BQ30, CARA_TERTT;  Q9HK16, CARA_THEAC;  Q9WZ28, CARA_THEMA;  
Q72GZ0, CARA_THET2;  Q5SLL3, CARA_THET8;  Q8DKU5, CARA_THEVB;  
Q97AJ4, CARA_THEVO;  B8GNX4, CARA_THISH;  Q9KPH8, CARA_VIBCH;  
Q87SF4, CARA_VIBPA;  Q8DEM1, CARA_VIBVU;  Q7MNU1, CARA_VIBVY;  
Q8D3H7, CARA_WIGBR;  P58895, CARA_XANAC;  P58896, CARA_XANCP;  
Q9PEC2, CARA_XYLFA;  Q87EB9, CARA_XYLFT;  Q6C9Y4, CARA_YARLI;  
P07258, CARA_YEAST;  Q8ZIL5, CARA_YERPE;  Q66ER8, CARA_YERPS;  
O50235, CARA_ZYMMO;  O67869, CARB1_AQUAE;  Q58773, CARB1_METJA;  
Q8TWX0, CARB1_METKA;  O67233, CARB2_AQUAE;  Q58776, CARB2_METJA;  
Q8TUT7, CARB2_METKA;  C1F1S6, CARB_ACIC5;  C4ZEK2, CARB_AGARV;  
Q8UDE9, CARB_AGRFC;  Q42601, CARB_ARATH;  O28994, CARB_ARCFU;  
A0JX72, CARB_ARTS2;  Q75D66, CARB_ASHGO;  C3P656, CARB_BACAA;  
C3L740, CARB_BACAC;  A0RHQ8, CARB_BACAH;  Q81WF2, CARB_BACAN;  
B7JJX3, CARB_BACC0;  Q732I3, CARB_BACC1;  B7IUP6, CARB_BACC2;  
C1EPQ0, CARB_BACC3;  B7H6M2, CARB_BACC4;  B7HLM0, CARB_BACC7;  
P46537, CARB_BACCL;  A7GRL1, CARB_BACCN;  B9IVW3, CARB_BACCQ;  
Q819S3, CARB_BACCR;  Q636E0, CARB_BACCZ;  Q6HES8, CARB_BACHK;  
A9VTC6, CARB_BACMK;  P25994, CARB_BACSU;  C5C687, CARB_BEUC1;  
B8DTW3, CARB_BIFA0;  A1A0T4, CARB_BIFAA;  B3DQ32, CARB_BIFLD;  
Q8G815, CARB_BIFLO;  B7GPW2, CARB_BIFLS;  Q8YIC2, CARB_BRUME;  
Q8FZJ3, CARB_BRUSU;  P57244, CARB_BUCAI;  Q8K9Z7, CARB_BUCAP;  
P59448, CARB_BUCBP;  Q8RBK0, CARB_CALS4;  Q9PIL7, CARB_CAMJE;  
Q9A4D6, CARB_CAUVC;  A5CRX5, CARB_CLAM3;  B0REV9, CARB_CLAMS;  
Q97FT3, CARB_CLOAB;  A6LPD9, CARB_CLOB8;  B2UX86, CARB_CLOBA;  
B2THH3, CARB_CLOBB;  C1FNY3, CARB_CLOBJ;  A7GE89, CARB_CLOBL;  
B1KT07, CARB_CLOBM;  Q0TM79, CARB_CLOP1;  Q8XHB3, CARB_CLOPE;  
Q0SPY4, CARB_CLOPS;  Q8FT42, CARB_COREF;  P58939, CARB_CORGL;  
Q6A914, CARB_CUTAK;  P46056, CARB_CUTCT;  C1CXR4, CARB_DEIDV;  
Q1IWM0, CARB_DEIGD;  Q9RWK0, CARB_DEIRA;  B1I4M8, CARB_DESAP;  
P63737, CARB_ECO57;  Q8FLB0, CARB_ECOL6;  P00968, CARB_ECOLI;  
Q834E2, CARB_ENTFA;  B1YIR9, CARB_EXIS2;  C4L5W3, CARB_EXISA;  
Q8RG86, CARB_FUSNN;  C1A4I5, CARB_GEMAT;  O50302, CARB_GEOSE;  
Q7VP67, CARB_HAEDU;  Q8RSS3, CARB_HALER;  Q9K9V9, CARB_HALH5;  
B8D1H3, CARB_HALOH;  Q9HP43, CARB_HALSA;  Q9ZKT2, CARB_HELPJ;  
O25577, CARB_HELPY;  A2BJL3, CARB_HYPBU;  A6WCC6, CARB_KINRD;  
B2GI87, CARB_KOCRD;  Q1IPK2, CARB_KORVE;  Q5FJC0, CARB_LACAC;  
B3WEF5, CARB_LACCB;  Q047M8, CARB_LACDB;  A8YVZ3, CARB_LACH4;  
Q74J34, CARB_LACJO;  Q9CFV2, CARB_LACLA;  O32771, CARB_LACLM;  
Q02YG5, CARB_LACLS;  Q038Z2, CARB_LACP3;  A9KI94, CARB_LACP7;  
P77886, CARB_LACPL;  Q38X24, CARB_LATSS;  Q72NF1, CARB_LEPIC;  
Q8F832, CARB_LEPIN;  B1MZ74, CARB_LEUCK;  Q03WW7, CARB_LEUMM;  
Q1WVA9, CARB_LIGS1;  B2GD06, CARB_LIMF3;  A5VHN6, CARB_LIMRD;  
B2G564, CARB_LIMRJ;  Q92AH3, CARB_LISIN;  C1KWD4, CARB_LISMC;  
Q71YI1, CARB_LISMF;  B8DDR7, CARB_LISMH;  Q8Y665, CARB_LISMO;  
A0AJU0, CARB_LISW6;  B1HQC1, CARB_LYSSC;  B9EB69, CARB_MACCJ;  
Q8TNY4, CARB_METAC;  A7I6J1, CARB_METB6;  P13258, CARB_METBA;  
Q469Z7, CARB_METBF;  Q2FLD9, CARB_METHJ;  A2SQ53, CARB_METLZ;  
P58944, CARB_METMA;  A3CU73, CARB_METMJ;  B8GEA3, CARB_METPE;  
A5UKG5, CARB_METS3;  O27077, CARB_METTH;  A0B8K9, CARB_METTP;  
C5CCF1, CARB_MICLC;  Q7U054, CARB_MYCBO;  A4TBY6, CARB_MYCGI;  
Q9CCR2, CARB_MYCLE;  A3PZ65, CARB_MYCSJ;  A1UFK4, CARB_MYCSK;  
Q1B9F9, CARB_MYCSS;  P9WPK2, CARB_MYCTO;  P9WPK3, CARB_MYCTU;  
A1T8H1, CARB_MYCVP;  Q1D6Y8, CARB_MYXXD;  B2A170, CARB_NATTJ;  
Q59599, CARB_NEIGO;  Q9JW02, CARB_NEIMA;  Q9JXW8, CARB_NEIMB;  
Q8YQL2, CARB_NOSS1;  Q04H29, CARB_OENOB;  B9EXM2, CARB_ORYSJ;  
A1R6Z3, CARB_PAEAT;  Q9CKV0, CARB_PASMU;  Q03HB0, CARB_PEDPA;  
A5D508, CARB_PELTS;  P38100, CARB_PSEAE;  B8H8U5, CARB_PSECP;  
A8F453, CARB_PSELT;  Q88DU6, CARB_PSEPK;  Q87WP4, CARB_PSESM;  
Q8ZY48, CARB_PYRAE;  Q8U085, CARB_PYRFU;  Q8XZ83, CARB_RALSO;  
A9WSA8, CARB_RENSM;  Q98I87, CARB_RHILO;  Q92PZ4, CARB_RHIME;  
Q7UJ58, CARB_RHOBA;  Q1AVY9, CARB_RUBXD;  Q59969, CARB_SACS2;  
Q8Z9L7, CARB_SALTI;  P14846, CARB_SALTY;  O94313, CARB_SCHPO;  
P63738, CARB_SHIFL;  A7X1F4, CARB_STAA1;  A6U122, CARB_STAA2;  
Q2FHN5, CARB_STAA3;  Q2FZ72, CARB_STAA8;  A5IS88, CARB_STAA9;  
Q2YXG5, CARB_STAAB;  Q5HGM9, CARB_STAAC;  A6QGA4, CARB_STAAE;  
P63739, CARB_STAAM;  P63740, CARB_STAAN;  Q6GHN2, CARB_STAAR;  
Q6GA10, CARB_STAAS;  A8Z3P0, CARB_STAAT;  P58940, CARB_STAAW;  
B9DPN2, CARB_STACT;  Q5HPY8, CARB_STAEQ;  Q8CPJ4, CARB_STAES;  
Q4L5Q5, CARB_STAHJ;  Q49WY4, CARB_STAS1;  Q3K150, CARB_STRA1;  
Q8E5F5, CARB_STRA3;  Q8DZQ7, CARB_STRA5;  Q827Q7, CARB_STRAW;  
Q9KXR6, CARB_STRCO;  C0M756, CARB_STRE4;  A8AX83, CARB_STRGC;  
B1W463, CARB_STRGG;  Q8DUP3, CARB_STRMU;  Q9A0C6, CARB_STRP1;  
Q04K48, CARB_STRP2;  P0DA14, CARB_STRP3;  B5E512, CARB_STRP4;  
Q5XCR6, CARB_STRP6;  C1C7Q3, CARB_STRP7;  P58941, CARB_STRP8;  
A2RF60, CARB_STRPG;  B1IC65, CARB_STRPI;  B8ZJT9, CARB_STRPJ;  
Q97QE4, CARB_STRPN;  P0DA15, CARB_STRPQ;  B2IQ67, CARB_STRPS;  
B5XKW2, CARB_STRPZ;  Q8CWR0, CARB_STRR6;  C0MEH1, CARB_STRS7;  
A3CNI5, CARB_STRSV;  Q5M0W9, CARB_STRT1;  Q5M5F6, CARB_STRT2;  
Q03LT8, CARB_STRTD;  B9DRV7, CARB_STRU0;  C1CEM9, CARB_STRZJ;  
C1CL09, CARB_STRZP;  C1CR31, CARB_STRZT;  Q4J8E8, CARB_SULAC;  
C3N664, CARB_SULIA;  C4KHM7, CARB_SULIK;  C3MQE3, CARB_SULIL;  
C3MW21, CARB_SULIM;  C3NH23, CARB_SULIN;  C3NEM0, CARB_SULIY;  
Q970U7, CARB_SULTO;  Q67Q54, CARB_SYMTH;  Q55756, CARB_SYNY3;  
Q9HK17, CARB_THEAC;  Q9WZ27, CARB_THEMA;  B9KB91, CARB_THENN;  
A5IJL8, CARB_THEP1;  B0KBW4, CARB_THEP3;  B0K4D7, CARB_THEPX;  
B9KXM5, CARB_THERP;  B1L8T8, CARB_THESQ;  P96495, CARB_THET2;  
Q5SKN1, CARB_THET8;  Q97AJ3, CARB_THEVO;  Q9KPH9, CARB_VIBCH;  
Q87SF3, CARB_VIBPA;  Q8DEM2, CARB_VIBVU;  Q7MNU0, CARB_VIBVY;  
P58942, CARB_XANAC;  P58943, CARB_XANCP;  Q9PEC1, CARB_XYLFA;  
Q87EB8, CARB_XYLFT;  P03965, CARB_YEAST;  Q8ZIL4, CARB_YERPE;  
O50236, CARB_ZYMMO;  P36838, CARX_BACSU;  P54324, CARX_GEOSE;  
Q9K8V6, CARX_HALH5;  O08317, CARX_LACPL;  P18185, CARY_BACSU;  
Q9ZB63, CARY_GEOSE;  Q9K8V7, CARY_HALH5;  Q9RLS9, CARY_LACPL;  
Q18990, PYR1_CAEEL;  P20054, PYR1_DICDI;  P05990, PYR1_DROME;  
O93937, PYR1_EMENI;  P27708, PYR1_HUMAN;  P08955, PYR1_MESAU;  
B2RQC6, PYR1_MOUSE;  Q09794, PYR1_SCHPO;  Q91437, PYR1_SQUAC;  
P07259, PYR1_YEAST;  

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