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ENZYME

ENZYME entry: EC 6.3.5.5

Accepted Name
carbamoyl-phosphate synthase (glutamine-hydrolyzing)
Alternative Name(s)
carbamoyl phosphate synthetase
carbamoyl phosphate synthetase (glutamine)
carbamoyl-phosphate synthetase (glutamine-hydrolyzing)
carbamoylphosphate synthetase II
CPS
GD-CPSase
glutamine-dependent carbamoyl-phosphate synthase
glutamine-dependent carbamoyl phosphate synthetase
Reaction catalysed
2 ATP + H2O + hydrogencarbonate + L-glutamine <=> 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate
Comment(s)
  • The product carbamoyl phosphate is an intermediate in the biosynthesis of arginine and the pyrimidine nucleotides.
  • The enzyme from Escherichia coli has three separate active sites, which are connected by a molecular tunnel that is almost 100 A in length.
  • The amidotransferase domain within the small subunit of the enzyme hydrolyzes glutamine to ammonia via a thioester intermediate.
  • The ammonia migrates through the interior of the protein, where it reacts with carboxyphosphate to produce the carbamate intermediate.
  • The carboxyphosphate intermediate is formed by the phosphorylation of hydrogencarbonate by ATP at a site contained within the N-terminal half of the large subunit.
  • The carbamate intermediate is transported through the interior of the protein to a second site within the C-terminal half of the large subunit, where it is phosphorylated by another ATP to yield the final product, carbamoyl phosphate. cf. EC 6.3.4.16.
  • Formerly EC 2.7.2.9.
Cross-references
BRENDA6.3.5.5
EC2PDB6.3.5.5
ExplorEnz6.3.5.5
PRIAM enzyme-specific profiles6.3.5.5
KEGG Ligand Database for Enzyme Nomenclature6.3.5.5
IUBMB Enzyme Nomenclature6.3.5.5
IntEnz6.3.5.5
MEDLINEFind literature relating to 6.3.5.5
MetaCyc6.3.5.5
Rhea expert-curated reactions6.3.5.5
UniProtKB/Swiss-Prot
Q6F8M7, CARA_ACIADQ8UDF7, CARA_AGRFCB2URJ0, CARA_AKKM8
O66727, CARA_AQUAEQ9LVW7, CARA_ARATHO28995, CARA_ARCFU
Q752N9, CARA_ASHGOA1CA18, CARA_ASPCLQ4WU09, CARA_ASPFU
A2R9B9, CARA_ASPNCQ2U913, CARA_ASPORQ81WF1, CARA_BACAN
Q732I2, CARA_BACC1P52557, CARA_BACCLQ819S2, CARA_BACCR
Q636D9, CARA_BACCZQ6HES7, CARA_BACHKP25993, CARA_BACSU
A1UTE8, CARA_BARBKQ6G5M0, CARA_BARHEQ6FZ66, CARA_BARQU
A9IWF8, CARA_BART1B3DQ31, CARA_BIFLDQ8G816, CARA_BIFLO
B7GPW1, CARA_BIFLSQ89DR8, CARA_BRADUB2S6U9, CARA_BRUA1
Q2YM22, CARA_BRUA2A6WZJ5, CARA_BRUA4Q57C28, CARA_BRUAB
A9M6F1, CARA_BRUC2C0REC2, CARA_BRUMBQ8YIB8, CARA_BRUME
A5VRM1, CARA_BRUO2B0CHS0, CARA_BRUSIQ8FZJ8, CARA_BRUSU
P57245, CARA_BUCAIQ8K9Z6, CARA_BUCAPP59576, CARA_BUCBP
Q8RBK1, CARA_CALS4Q9PMG8, CARA_CAMJEQ5AML6, CARA_CANAL
Q6FQ30, CARA_CANGBQ9A4J7, CARA_CAUVCQ2H132, CARA_CHAGB
Q8KGA2, CARA_CHLTEQ97FT2, CARA_CLOABQ8XHB2, CARA_CLOPE
Q1DUF5, CARA_COCIMQ6NH15, CARA_CORDIQ8FT41, CARA_COREF
P58893, CARA_CORGLO19886, CARA_CYACAQ6BJG4, CARA_DEBHA
Q1IWN0, CARA_DEIGDQ9RWI4, CARA_DEIRAB8IZS6, CARA_DESDA
Q726J4, CARA_DESVHQ8KZA0, CARA_DESVMA1VA26, CARA_DESVV
P0A6F2, CARA_ECO57Q8FLB1, CARA_ECOL6P0A6F1, CARA_ECOLI
Q5BB37, CARA_EMENIQ8RG87, CARA_FUSNNQ6B940, CARA_GRATL
Q7VP66, CARA_HAEDUQ8RSS4, CARA_HALERQ9K9V8, CARA_HALH5
B0R6F1, CARA_HALS3Q9HP42, CARA_HALSAQ17VI1, CARA_HELAH
Q7VHS5, CARA_HELHPQ9ZJY9, CARA_HELPJO25835, CARA_HELPY
Q0BY73, CARA_HYPNAP87183, CARA_HYPVIQ6CIQ1, CARA_KLULC
Q9CF80, CARA_LACLAQ9L4N5, CARA_LACLMP77885, CARA_LACPL
Q1MPN3, CARA_LAWIPQ04R57, CARA_LEPBJQ04ZG6, CARA_LEPBL
Q72PK5, CARA_LEPICQ8F6R2, CARA_LEPINQ92AH2, CARA_LISIN
Q71YI0, CARA_LISMFQ8Y664, CARA_LISMOQ0AMR3, CARA_MARMM
C6C0A9, CARA_MARSDQ8TNY3, CARA_METACQ58425, CARA_METJA
Q8TY15, CARA_METKAA4G0Y3, CARA_METM5A9A972, CARA_METM6
A6VHH7, CARA_METM7Q8Q0U4, CARA_METMAQ6LWW5, CARA_METMP
A4YI77, CARA_METS5O27079, CARA_METTHA6UQN4, CARA_METVS
Q7U055, CARA_MYCBOQ9CCR3, CARA_MYCLEQ741H2, CARA_MYCPA
P9WPK4, CARA_MYCTOP9WPK5, CARA_MYCTUQ50983, CARA_NEIGO
Q9JVZ6, CARA_NEIMAQ9JXX4, CARA_NEIMBA1CZ92, CARA_NEOFI
Q1XDT6, CARA_NEOYEP22572, CARA_NEUCRQ8YXQ7, CARA_NOSS1
Q8CXH8, CARA_OCEIHQ6YV23, CARA_ORYSJQ7U7F9, CARA_PARMW
Q9CKV3, CARA_PASMUQ6D0C8, CARA_PECASQ0U5H7, CARA_PHANO
Q7N8W2, CARA_PHOLLQ6LUK6, CARA_PHOPRA4SXM1, CARA_POLAQ
B1XUM3, CARA_POLNSP51201, CARA_PORPUB4S5S1, CARA_PROA2
P38098, CARA_PSEAEQ88DU5, CARA_PSEPKQ87WP3, CARA_PSESM
Q8ZY49, CARA_PYRAEQ8U086, CARA_PYRFUQ8XZ85, CARA_RALSO
Q98IA7, CARA_RHILOQ92N95, CARA_RHIMEQ7UGJ6, CARA_RHOBA
Q9JP87, CARA_RUBGIQ5LTN6, CARA_RUEPOQ59968, CARA_SACS2
Q8Z9L8, CARA_SALTIP14845, CARA_SALTYO60060, CARA_SCHPO
Q8EHS6, CARA_SHEONC4XRH8, CARA_SOLM1Q5HGN0, CARA_STAAC
P63729, CARA_STAAMP99147, CARA_STAANQ6GHN3, CARA_STAAR
Q6GA11, CARA_STAASP63730, CARA_STAAWQ5HPY9, CARA_STAEQ
Q8CPJ5, CARA_STAESQ4L5Q4, CARA_STAHJQ49WY3, CARA_STAS1
P63731, CARA_STRA3P63732, CARA_STRA5Q827Q8, CARA_STRAW
Q9KXR5, CARA_STRCOQ8DUP4, CARA_STRMUP63735, CARA_STRP1
P0DA12, CARA_STRP3Q5XCR8, CARA_STRP6P58894, CARA_STRP8
P63733, CARA_STRPNP0DA13, CARA_STRPQP63734, CARA_STRR6
P38099, CARA_STUSTQ4J8E9, CARA_SULACQ970U8, CARA_SULTO
Q67Q55, CARA_SYMTHQ3A450, CARA_SYNC1Q31LB7, CARA_SYNE7
Q5N0L0, CARA_SYNP6A5GTM5, CARA_SYNR3P74587, CARA_SYNY3
C5BQ30, CARA_TERTTQ9HK16, CARA_THEACQ9WZ28, CARA_THEMA
Q72GZ0, CARA_THET2Q5SLL3, CARA_THET8Q8DKU5, CARA_THEVB
Q97AJ4, CARA_THEVOB8GNX4, CARA_THISHQ9KPH8, CARA_VIBCH
Q87SF4, CARA_VIBPAQ8DEM1, CARA_VIBVUQ7MNU1, CARA_VIBVY
Q8D3H7, CARA_WIGBRP58895, CARA_XANACP58896, CARA_XANCP
Q9PEC2, CARA_XYLFAQ87EB9, CARA_XYLFTQ6C9Y4, CARA_YARLI
P07258, CARA_YEASTQ8ZIL5, CARA_YERPEQ66ER8, CARA_YERPS
O50235, CARA_ZYMMOO67869, CARB1_AQUAEQ58773, CARB1_METJA
Q8TWX0, CARB1_METKAO67233, CARB2_AQUAEQ58776, CARB2_METJA
Q8TUT7, CARB2_METKAC1F1S6, CARB_ACIC5C4ZEK2, CARB_AGARV
Q8UDE9, CARB_AGRFCQ42601, CARB_ARATHO28994, CARB_ARCFU
A0JX72, CARB_ARTS2Q75D66, CARB_ASHGOC3P656, CARB_BACAA
C3L740, CARB_BACACA0RHQ8, CARB_BACAHQ81WF2, CARB_BACAN
B7JJX3, CARB_BACC0Q732I3, CARB_BACC1B7IUP6, CARB_BACC2
C1EPQ0, CARB_BACC3B7H6M2, CARB_BACC4B7HLM0, CARB_BACC7
P46537, CARB_BACCLA7GRL1, CARB_BACCNB9IVW3, CARB_BACCQ
Q819S3, CARB_BACCRQ636E0, CARB_BACCZQ6HES8, CARB_BACHK
A9VTC6, CARB_BACMKP25994, CARB_BACSUC5C687, CARB_BEUC1
B8DTW3, CARB_BIFA0A1A0T4, CARB_BIFAAB3DQ32, CARB_BIFLD
Q8G815, CARB_BIFLOB7GPW2, CARB_BIFLSQ8YIC2, CARB_BRUME
Q8FZJ3, CARB_BRUSUP57244, CARB_BUCAIQ8K9Z7, CARB_BUCAP
P59448, CARB_BUCBPQ8RBK0, CARB_CALS4Q9PIL7, CARB_CAMJE
Q9A4D6, CARB_CAUVCA5CRX5, CARB_CLAM3B0REV9, CARB_CLAMS
Q97FT3, CARB_CLOABA6LPD9, CARB_CLOB8B2UX86, CARB_CLOBA
B2THH3, CARB_CLOBBC1FNY3, CARB_CLOBJA7GE89, CARB_CLOBL
B1KT07, CARB_CLOBMQ0TM79, CARB_CLOP1Q8XHB3, CARB_CLOPE
Q0SPY4, CARB_CLOPSQ8FT42, CARB_COREFP58939, CARB_CORGL
Q6A914, CARB_CUTAKP46056, CARB_CUTCTC1CXR4, CARB_DEIDV
Q1IWM0, CARB_DEIGDQ9RWK0, CARB_DEIRAB1I4M8, CARB_DESAP
P63737, CARB_ECO57Q8FLB0, CARB_ECOL6P00968, CARB_ECOLI
Q834E2, CARB_ENTFAB1YIR9, CARB_EXIS2C4L5W3, CARB_EXISA
Q8RG86, CARB_FUSNNC1A4I5, CARB_GEMATO50302, CARB_GEOSE
Q7VP67, CARB_HAEDUQ8RSS3, CARB_HALERQ9K9V9, CARB_HALH5
B8D1H3, CARB_HALOHQ9HP43, CARB_HALSAQ9ZKT2, CARB_HELPJ
O25577, CARB_HELPYA2BJL3, CARB_HYPBUA6WCC6, CARB_KINRD
B2GI87, CARB_KOCRDQ1IPK2, CARB_KORVEQ5FJC0, CARB_LACAC
B3WEF5, CARB_LACCBQ047M8, CARB_LACDBA8YVZ3, CARB_LACH4
Q74J34, CARB_LACJOQ9CFV2, CARB_LACLAO32771, CARB_LACLM
Q02YG5, CARB_LACLSQ038Z2, CARB_LACP3A9KI94, CARB_LACP7
P77886, CARB_LACPLQ38X24, CARB_LATSSQ72NF1, CARB_LEPIC
Q8F832, CARB_LEPINB1MZ74, CARB_LEUCKQ03WW7, CARB_LEUMM
Q1WVA9, CARB_LIGS1B2GD06, CARB_LIMF3A5VHN6, CARB_LIMRD
B2G564, CARB_LIMRJQ92AH3, CARB_LISINC1KWD4, CARB_LISMC
Q71YI1, CARB_LISMFB8DDR7, CARB_LISMHQ8Y665, CARB_LISMO
A0AJU0, CARB_LISW6B1HQC1, CARB_LYSSCB9EB69, CARB_MACCJ
Q8TNY4, CARB_METACA7I6J1, CARB_METB6P13258, CARB_METBA
Q469Z7, CARB_METBFQ2FLD9, CARB_METHJA2SQ53, CARB_METLZ
P58944, CARB_METMAA3CU73, CARB_METMJB8GEA3, CARB_METPE
A5UKG5, CARB_METS3O27077, CARB_METTHA0B8K9, CARB_METTP
C5CCF1, CARB_MICLCQ7U054, CARB_MYCBOA4TBY6, CARB_MYCGI
Q9CCR2, CARB_MYCLEA3PZ65, CARB_MYCSJA1UFK4, CARB_MYCSK
Q1B9F9, CARB_MYCSSP9WPK2, CARB_MYCTOP9WPK3, CARB_MYCTU
A1T8H1, CARB_MYCVPQ1D6Y8, CARB_MYXXDB2A170, CARB_NATTJ
Q59599, CARB_NEIGOQ9JW02, CARB_NEIMAQ9JXW8, CARB_NEIMB
Q8YQL2, CARB_NOSS1Q04H29, CARB_OENOBB9EXM2, CARB_ORYSJ
A1R6Z3, CARB_PAEATQ9CKV0, CARB_PASMUQ03HB0, CARB_PEDPA
A5D508, CARB_PELTSP38100, CARB_PSEAEB8H8U5, CARB_PSECP
A8F453, CARB_PSELTQ88DU6, CARB_PSEPKQ87WP4, CARB_PSESM
Q8ZY48, CARB_PYRAEQ8U085, CARB_PYRFUQ8XZ83, CARB_RALSO
A9WSA8, CARB_RENSMQ98I87, CARB_RHILOQ92PZ4, CARB_RHIME
Q7UJ58, CARB_RHOBAQ1AVY9, CARB_RUBXDQ59969, CARB_SACS2
Q8Z9L7, CARB_SALTIP14846, CARB_SALTYO94313, CARB_SCHPO
P63738, CARB_SHIFLA7X1F4, CARB_STAA1A6U122, CARB_STAA2
Q2FHN5, CARB_STAA3Q2FZ72, CARB_STAA8A5IS88, CARB_STAA9
Q2YXG5, CARB_STAABQ5HGM9, CARB_STAACA6QGA4, CARB_STAAE
P63739, CARB_STAAMP63740, CARB_STAANQ6GHN2, CARB_STAAR
Q6GA10, CARB_STAASA8Z3P0, CARB_STAATP58940, CARB_STAAW
B9DPN2, CARB_STACTQ5HPY8, CARB_STAEQQ8CPJ4, CARB_STAES
Q4L5Q5, CARB_STAHJQ49WY4, CARB_STAS1Q3K150, CARB_STRA1
Q8E5F5, CARB_STRA3Q8DZQ7, CARB_STRA5Q827Q7, CARB_STRAW
Q9KXR6, CARB_STRCOC0M756, CARB_STRE4A8AX83, CARB_STRGC
B1W463, CARB_STRGGQ8DUP3, CARB_STRMUQ9A0C6, CARB_STRP1
Q04K48, CARB_STRP2P0DA14, CARB_STRP3B5E512, CARB_STRP4
Q5XCR6, CARB_STRP6C1C7Q3, CARB_STRP7P58941, CARB_STRP8
A2RF60, CARB_STRPGB1IC65, CARB_STRPIB8ZJT9, CARB_STRPJ
Q97QE4, CARB_STRPNP0DA15, CARB_STRPQB2IQ67, CARB_STRPS
B5XKW2, CARB_STRPZQ8CWR0, CARB_STRR6C0MEH1, CARB_STRS7
A3CNI5, CARB_STRSVQ5M0W9, CARB_STRT1Q5M5F6, CARB_STRT2
Q03LT8, CARB_STRTDB9DRV7, CARB_STRU0C1CEM9, CARB_STRZJ
C1CL09, CARB_STRZPC1CR31, CARB_STRZTQ4J8E8, CARB_SULAC
C3N664, CARB_SULIAC4KHM7, CARB_SULIKC3MQE3, CARB_SULIL
C3MW21, CARB_SULIMC3NH23, CARB_SULINC3NEM0, CARB_SULIY
Q970U7, CARB_SULTOQ67Q54, CARB_SYMTHQ55756, CARB_SYNY3
Q9HK17, CARB_THEACQ9WZ27, CARB_THEMAB9KB91, CARB_THENN
A5IJL8, CARB_THEP1B0KBW4, CARB_THEP3B0K4D7, CARB_THEPX
B9KXM5, CARB_THERPB1L8T8, CARB_THESQP96495, CARB_THET2
Q5SKN1, CARB_THET8Q97AJ3, CARB_THEVOQ9KPH9, CARB_VIBCH
Q87SF3, CARB_VIBPAQ8DEM2, CARB_VIBVUQ7MNU0, CARB_VIBVY
P58942, CARB_XANACP58943, CARB_XANCPQ9PEC1, CARB_XYLFA
Q87EB8, CARB_XYLFTP03965, CARB_YEASTQ8ZIL4, CARB_YERPE
O50236, CARB_ZYMMOP36838, CARX_BACSUP54324, CARX_GEOSE
Q9K8V6, CARX_HALH5O08317, CARX_LACPLP18185, CARY_BACSU
Q9ZB63, CARY_GEOSEQ9K8V7, CARY_HALH5Q9RLS9, CARY_LACPL
Q18990, PYR1_CAEELP20054, PYR1_DICDIP05990, PYR1_DROME
O93937, PYR1_EMENIP27708, PYR1_HUMANP08955, PYR1_MESAU
B2RQC6, PYR1_MOUSEQ09794, PYR1_SCHPOQ91437, PYR1_SQUAC
P07259, PYR1_YEAST

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All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.3.5.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.3.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-