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ENZYME

ENZYME entry: EC 6.3.5.11

PURL: https://purl.expasy.org/enzyme/EC/6.3.5.11
Accepted Name
cobyrinate a,c-diamide synthase (glutamine-hydrolyzing)
Alternative Name(s)
cobyrinic acid a,c-diamide synthetase
Reaction catalysed
cob(II)yrinate + 2 L-glutamine + 2 ATP + 2 H2O = cob(II)yrinate a,c diamide + 2 L-glutamate + 2 ADP + 2 phosphate + 2 H(+)
Comment(s)
  • This enzyme is the first glutamine amidotransferase that participates in the anaerobic (early cobalt insertion) biosynthetic pathway of adenosylcobalamin, and catalyzes the ATP-dependent synthesis of cobyrinate a,c-diamide from cobyrinate using either L-glutamine or ammonia as the nitrogen source.
  • It is proposed that the enzyme first catalyzes the amidation of the c-carboxylate, and then the intermediate is released into solution and binds to the same catalytic site for the amidation of the a-carboxylate.
  • The Km for ammonia is substantially higher than that for L-glutamine.
  • The equivalent reaction in the aerobic cobalamin biosynthesis pathway is catalyzed by EC 6.3.5.9.
Cross-references
BRENDA6.3.5.11
EC2PDB6.3.5.11
ExplorEnz6.3.5.11
KEGG Ligand Database for Enzyme Nomenclature6.3.5.11
IUBMB Enzyme Nomenclature6.3.5.11
MEDLINEFind literature relating to 6.3.5.11
MetaCyc6.3.5.11
Rhea expert-curated reactions6.3.5.11
UniProtKB/Swiss-Prot
O28054, CBIA_ARCFUQ8RCP4, CBIA_CALS4Q97JB1, CBIA_CLOAB
Q897K3, CBIA_CLOTEQ3ZA71, CBIA_DEHM1Q3ZWJ9, CBIA_DEHMC
Q8R659, CBIA_FUSNNQ7NF32, CBIA_GLOVIQ9KBM8, CBIA_HALH5
Q5V341, CBIA_HALMAQ9HPL7, CBIA_HALSAA4G3R1, CBIA_HERAR
Q75FQ8, CBIA_LEPICQ8EXQ4, CBIA_LEPINA5VM87, CBIA_LIMRD
B2G9J4, CBIA_LIMRJQ92CL7, CBIA_LISINQ720N8, CBIA_LISMF
Q8Y7T0, CBIA_LISMOA0L8B9, CBIA_MAGMMQ8TK00, CBIA_METAC
Q46FL0, CBIA_METBFQ58816, CBIA_METJAA4FW46, CBIA_METM5
A6VH63, CBIA_METM7A5UMJ2, CBIA_METS3Q2NHQ3, CBIA_METST
O27509, CBIA_METTHA6UQC1, CBIA_METVSA1AT17, CBIA_PELPD
Q6L2V8, CBIA_PICTOO87698, CBIA_PRIMGQ980B1, CBIA_SACS2
P29946, CBIA_SALTYA3CL56, CBIA_STRSVQ4JAI7, CBIA_SULAC
Q975N0, CBIA_SULTOQ3A7A3, CBIA_SYNC1Q5MZU1, CBIA_SYNP6
P73002, CBIA_SYNY3Q9HIX6, CBIA_THEACQ97BP4, CBIA_THEVO

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