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ENZYME

ENZYME entry: EC 6.3.5.4

Accepted Name
asparagine synthase (glutamine-hydrolyzing)
Alternative Name(s)
AS-B
asparagine synthetase (glutamine-hydrolyzing)
asparagine synthetase B
glutamine-dependent asparagine synthetase
Reaction catalysed
ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate
Comment(s)
  • The enzyme from Escherichia coli has two active sites that are connected by an intramolecular ammonia tunnel.
  • The enzyme catalyzes three distinct chemical reactions: glutamine hydrolysis to yield ammonia takes place in the N-terminal domain.
  • The C-terminal active site mediates both the synthesis of a beta- aspartyl-AMP intermediate and its subsequent reaction with ammonia.
  • The ammonia released is channeled to the other active site to yield asparagine.
Cross-references
BRENDA6.3.5.4
EC2PDB6.3.5.4
ExplorEnz6.3.5.4
PRIAM enzyme-specific profiles6.3.5.4
KEGG Ligand Database for Enzyme Nomenclature6.3.5.4
IUBMB Enzyme Nomenclature6.3.5.4
IntEnz6.3.5.4
MEDLINEFind literature relating to 6.3.5.4
MetaCyc6.3.5.4
Rhea expert-curated reactions6.3.5.4
UniProtKB/Swiss-Prot
P54420, ASNB_BACSUP22106, ASNB_ECOLIQ58516, ASNH1_METJA
Q58456, ASNH2_METJAP42113, ASNH_BACSUP64248, ASNH_MYCBO
P9WN32, ASNH_MYCTOP9WN33, ASNH_MYCTUO05272, ASNO_BACSU
P49078, ASNS1_ARATHP49092, ASNS1_LOTJAQ10MX3, ASNS1_ORYSJ
P19251, ASNS1_PEAP49089, ASNS1_YEASTQ9LV77, ASNS2_ARATH
P49093, ASNS2_LOTJAQ43011, ASNS2_ORYSJP19252, ASNS2_PEA
P49090, ASNS2_YEASTQ9LFU1, ASNS3_ARATHP31752, ASNS_ASPOF
Q1LZA3, ASNS_BOVINP49091, ASNS_BRAOLQ5ZJU3, ASNS_CHICK
P19891, ASNS_CRIGRQ54MB4, ASNS_DICDIP08243, ASNS_HUMAN
P49094, ASNS_MAIZEP17714, ASNS_MESAUQ5UQE1, ASNS_MIMIV
Q61024, ASNS_MOUSEQ5R6W9, ASNS_PONABP49088, ASNS_RAT
O24338, ASNS_SANAUP78753, ASNS_SCHPOO24661, ASNS_TRIVS

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