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ENZYME

ENZYME entry: EC 1.5.1.53

Accepted Name
methylenetetrahydrofolate reductase (NADPH)
Alternative Name(s)
5,10-CH2-H4folate reductase
5,10-methylenetetrahydrofolate reductase (FADH2)
5,10-methylenetetrahydrofolate reductase (NADPH)
N(5),10-methylenetetrahydrofolate reductase
Reaction catalysed
(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH
Comment(s)
  • A flavoprotein (FAD).
  • The enzyme from yeast and mammals catalyzes a physiologically irreversible reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate using NADPH as the electron donor.
  • It plays an important role in folate metabolism by regulating the distribution of one-carbon moieties between cellular methylation reactions and nucleic acid synthesis.
  • The enzyme contains an N-terminal catalytic domain and a C-terminal allosteric regulatory domain that binds S-adenosyl-L-methionine, which acts as an inhibitor. cf. EC 1.5.1.54, EC 1.5.1.20, and EC 1.5.7.1.
Cross-references
BRENDA1.5.1.53
EC2PDB1.5.1.53
ExplorEnz1.5.1.53
PRIAM enzyme-specific profiles1.5.1.53
KEGG Ligand Database for Enzyme Nomenclature1.5.1.53
IUBMB Enzyme Nomenclature1.5.1.53
IntEnz1.5.1.53
MEDLINEFind literature relating to 1.5.1.53
MetaCyc1.5.1.53
Rhea expert-curated reactions1.5.1.53
UniProtKB/Swiss-Prot
O74927, MTHR2_SCHPOP53128, MTHR2_YEASTQ5I598, MTHR_BOVIN
Q17693, MTHR_CAEELP42898, MTHR_HUMANQ60HE5, MTHR_MACFA
Q9WU20, MTHR_MOUSE

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.5.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.5.-.-
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