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ENZYME

ENZYME entry: EC 4.1.99.24

PURL: https://purl.expasy.org/enzyme/EC/4.1.99.24
Accepted Name
L-tyrosine isonitrile synthase
Reaction catalysed
D-ribulose 5-phosphate + L-tyrosine = (2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate + hydroxyacetone + formaldehyde + phosphate + H2O + H(+)
Comment(s)
  • The enzymes from the bacteria Pseudomonas aeruginosa and Xenorhabdus nematophila are involved in the biosynthesis of paerucumarin and rhabduscin, respectively.
  • According to the proposed mechanism, the enzyme forms an imine intermediate composed of linked L-tyrosine and D-ribulose 5-phosphate, followed by loss of the phosphate group and formation of a beta-keto imine and keto-enol tautomerization.
  • This is followed by a C-C bond cleavage, the release of acetol, and a retro aldol type reaction that releases formaldehyde and forms the final product. cf. EC 4.1.99.25.
Cross-references
BRENDA4.1.99.24
EC2PDB4.1.99.24
ExplorEnz4.1.99.24
KEGG Ligand Database for Enzyme Nomenclature4.1.99.24
IUBMB Enzyme Nomenclature4.1.99.24
MEDLINEFind literature relating to 4.1.99.24
MetaCyc4.1.99.24
Rhea expert-curated reactions4.1.99.24
UniProtKB/Swiss-Prot
Q9I1L5, PVCA_PSEAED3V9Q4, PVCA_XENNA

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