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ENZYME entry: EC

Accepted Name
Asparaginyl-tRNA synthase (glutamine-hydrolyzing).
Alternative Name(s)
Asp-tRNA(Asn) amidotransferase.
Aspartyl-tRNA(Asn) amidotransferase.
Reaction catalysed
ATP + L-aspartyl-tRNA(Asn) + L-glutamine + H(2)O <=> ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate
  • This reaction forms part of a two-reaction system for producing asparaginyl-tRNA in Deinococcus radiodurans and other organisms lacking a specific enzyme for asparagine synthesis.
  • In the first step, a non-discriminating ligase (EC mischarges tRNA(Asn) with aspartate, leading to the formation of aspartyl-tRNA(Asn).
  • The aspartyl-tRNA(Asn) is not used in protein synthesis until the present enzyme converts it into asparaginyl-tRNA(Asn) (aspartyl- tRNA(Asp) is not a substrate for this enzyme).
  • A glutaminase subunit (cf. EC produces an ammonia molecule that is transferred by a 30 A tunnel to a synthase subunit, where it is ligated to the carboxy group that has been activated by phosphorylation.
  • Bacterial GatCAB complexes also has the activity of EC
PRIAM enzyme-specific profiles6.3.5.6
KEGG Ligand Database for Enzyme Nomenclature6.3.5.6
IUBMB Enzyme Nomenclature6.3.5.6
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