ENZYME entry: EC 6.3.5.6
| Accepted Name |
|---|
| Asparaginyl-tRNA synthase (glutamine-hydrolyzing).
|
| Alternative Name(s) |
|---|
| Asp-AdT. |
| Asp-tRNA(Asn) amidotransferase. |
| Aspartyl-tRNA(Asn) amidotransferase. |
| GatCAB. |
| Reaction catalysed |
|---|
| ATP + L-aspartyl-tRNA(Asn) + L-glutamine + H(2)O <=> ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate |
| Comment(s) |
|---|
- This reaction forms part of a two-reaction system for producing
asparaginyl-tRNA in Deinococcus radiodurans and other organisms
lacking a specific enzyme for asparagine synthesis.
- In the first step, a non-discriminating ligase (EC 6.1.1.23)
mischarges tRNA(Asn) with aspartate, leading to the formation of
aspartyl-tRNA(Asn).
- The aspartyl-tRNA(Asn) is not used in protein synthesis until the
present enzyme converts it into asparaginyl-tRNA(Asn) (aspartyl-
tRNA(Asp) is not a substrate for this enzyme).
- A glutaminase subunit (cf. EC 3.5.1.2) produces an ammonia molecule
that is transferred by a 30 A tunnel to a synthase subunit, where it
is ligated to the carboxy group that has been activated by
phosphorylation.
- Bacterial GatCAB complexes also has the activity of EC 6.3.5.7.
|
| Cross-references |
|---|
| BRENDA | 6.3.5.6 |
| EC2PDB | 6.3.5.6 |
| ExplorEnz | 6.3.5.6 |
| PRIAM enzyme-specific profiles | 6.3.5.6 |
| KEGG Ligand Database for Enzyme Nomenclature | 6.3.5.6 |
| IUBMB Enzyme Nomenclature | 6.3.5.6 |
| IntEnz | 6.3.5.6 |
| MEDLINE | Find literature relating to 6.3.5.6 |
| MetaCyc | 6.3.5.6 |
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