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ENZYME entry: EC

Accepted Name
glycine cleavage system
Alternative Name(s)
Reaction catalysed
(6S)-5,6,7,8-tetrahydrofolate + glycine + NAD(+) <=> (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + CO2 + NADH + NH4(+)
  • The glycine cleavage (GCV) system is a large multienzyme complex that belongs to the 2-oxoacid dehydrogenase complex family, which also includes EC, EC, EC and EC
  • The GCV system catalyzes the reversible oxidation of glycine, yielding carbon dioxide, ammonia, 5,10-methylenetetrahydrofolate and a reduced pyridine nucleotide.
  • Tetrahydrofolate serves as a recipient for one-carbon units generated during glycine cleavage to form the methylene group.
  • The GCV system consists of four protein components, the P protein (EC, T protein (EC, L protein (EC, and the non-enzyme H protein (lipoyl-carrier protein).
  • The P protein catalyzes the pyridoxal phosphate-dependent liberation of CO2 from glycine, leaving a methylamine moiety.
  • The methylamine moiety is transferred to the lipoic acid group of the H protein, which is bound to the P protein prior to decarboxylation of glycine.
  • The T protein catalyzes the release of ammonia from the methylamine group and transfers the remaining C1 unit to tetrahydrofolate, forming 5,10-methylenetetrahydrofolate.
  • The L protein then oxidizes the lipoic acid component of the H protein and transfers the electrons to NAD(+), forming NADH.
PRIAM enzyme-specific profiles1.4.1.27
KEGG Ligand Database for Enzyme Nomenclature1.4.1.27
IUBMB Enzyme Nomenclature1.4.1.27
MEDLINEFind literature relating to
Rhea expert-curated reactions1.4.1.27

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