| Accepted Name |
|---|
| caspase-3
|
| Alternative Name(s) |
|---|
| apopain |
| CASP-3 |
| CPP32 |
| yama protein |
| Reaction catalysed |
|---|
| Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position |
| Comment(s) |
|---|
- Caspase-3 is an effector/executioner caspase, as are caspase-6
(EC 3.4.22.59) and caspase-7 (EC 3.4.22.60).
- These caspases are responsible for the proteolysis of the majority of
cellular polypeptides, [e.g. poly(ADP-ribose) polymerase (PARP)],
which leads to the apoptotic phenotype.
- Procaspase-3 can be activated by caspase-1 (EC 3.4.22.36), caspase-8
(EC 3.4.22.61), caspase-9 (EC 3.4.22.62) and caspase-10
(EC 3.4.22.63) as well as by the serine protease granzyme B.
- Caspase-3 can activate procaspase-2 (EC 3.4.22.55).
- Activation occurs by inter-domain cleavage followed by removal of the
N-terminal prodomain.
- While Asp-Glu-(Val/Ile)-Asp is thought to be the preferred cleavage
sequence, the enzyme can accommodate different residues at P2 and P3
of the substrate.
- Like caspase-2, a hydrophobic residue at P5 of caspase-3 leads to
more efficient hydrolysis, e.g. (Val/Leu)-Asp-Val-Ala-Asp-|- is a
better substrate than Asp-Val-Ala-Asp-|-.
- This is not the case for caspase-7.
- Belongs to peptidase family C14.
|
| Cross-references |
|---|
| BRENDA | 3.4.22.56 |
| EC2PDB | 3.4.22.56 |
| ExplorEnz | 3.4.22.56 |
| KEGG Ligand Database for Enzyme Nomenclature | 3.4.22.56 |
| IUBMB Enzyme Nomenclature | 3.4.22.56 |
| MEDLINE | Find literature relating to 3.4.22.56 |
| MetaCyc | 3.4.22.56 |
| Rhea expert-curated reactions | 3.4.22.56 |
| UniProtKB/Swiss-Prot |
| Q08DY9, CASP3_BOVIN | Q8MKI5, CASP3_CANLF | Q8MJU1, CASP3_FELCA |
| P42574, CASP3_HUMAN | Q2PFV2, CASP3_MACFA | Q60431, CASP3_MESAU |
| P70677, CASP3_MOUSE | Q5IS54, CASP3_PANTR | Q95ND5, CASP3_PIG |
| Q8MJC3, CASP3_RABIT | P55213, CASP3_RAT | Q5IS99, CASP3_SAIBB |
| P55866, CASP3_XENLA |
|
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UniProtKB/Swiss-Prot entries corresponding to 3.4.22.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.4.-.-
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